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author:

林晓栩 (林晓栩.) [1] | 林新坚 (林新坚.) [2] | 邱宏端 (邱宏端.) [3] | 陈龙军 (陈龙军.) [4] | 陈济琛 (陈济琛.) [5]

Indexed by:

PKU CSCD

Abstract:

以重组环糊精葡萄糖基转移酶(CGTase)表达菌株BL21(DE3)作为研究对象,比较IPTG和乳糖诱导对重组大肠杆菌表达CGTase量的影响,确定优化诱导方式。以Geobacillus CHB1的基因组DNA为模板,采用PCR技术克隆CGTase基因;利用分子操作技术构建携带有大肠杆菌Omp A信号肽的分泌型表达质粒p EASY-E2-Omp A-CGT,重组质粒热激转化至Escherichia coli BL21中进行表达;在摇瓶发酵条件下,分别以IPTG和乳糖作为诱导剂,通过SDSPAGE分析和测定胞外酶活,确定优化诱导方式。CGTase基因在Escherichia coli BL21中...

Keyword:

Geobacillus CHB1 IPTG 乳糖 环糊精葡萄糖基转移酶 诱导表达

Community:

  • [ 1 ] 福州大学生物科学与工程学院
  • [ 2 ] 福建省农业科学院土壤肥料研究所

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Source :

食品工业科技

Year: 2015

Issue: 17

Volume: 36

Page: 114-120,126

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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