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Abstract:
采用DEAE Sepharose离子交换层析、Butyl Sepharosc疏水层析和Blue Sepharose亲和层析等分离方法,从高产D-(-)-扁桃酸的酵母菌株BY1.1b中纯化得到电泳纯的D-(-)-扁桃酸脱氢酶.采用Sephacryl S-200分子筛层析柱测得该酶的相对分子质量约60 kDa,用SDS-PAGE电泳测得该酶亚基分子量为32 kDa,表明该酶属于同型二聚体.该酶的最适反应温度为30℃,最适反应pH为6.0.该酶在低于30℃,pH 5.5-8.0较为稳定.以NADH和苯乙酮酸为底物,其Km值分别为K(NADH)=99.2 μmol/L和Km(苯乙酮酸)=263.3 μmol/L.
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福州大学学报(自然科学版)
ISSN: 1000-2243
CN: 35-1337/N
Year: 2008
Issue: 6
Volume: 36
Page: 905-909
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count: -1
Chinese Cited Count:
30 Days PV: 1
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