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[目的]在绛红色小单孢菌G 1008 (Micromonospora purpureaG 1008)上构建genA基因缺失工程菌,通过分析其次级代谢产物的变化,推测genA基因功能.[方法]构建用于genA基因框内敲除的质粒pAB103,经接合转移导入绛红色小单孢菌G1008,安普抗性及PCR扩增筛选获得genA缺失工程菌GA1048.[结果]与出发菌G1008相比,工程菌GA1048不再合成庆大霉素C族组分,主要积累中间代谢产物庆大霉素A2.[结论]genA基因失活导致庆大霉素生物合成代谢流中断,暗示genA基因参与加洛糖胺C-3"位的氨甲基化.
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微生物学通报
ISSN: 0253-2654
CN: 11-1996/Q
Year: 2015
Issue: 2
Volume: 42
Page: 300-306
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count: -1
Chinese Cited Count:
30 Days PV: 6
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