Rolling circle amplification combined with gold nanoparticles-tag for ultra sensitive and specific quantification of DNA by inductively coupled plasma mass spectrometry - Details

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Abstract：

A　novel　method　for　the　ultra　specific　and　sensitive　detection　of　DNA　in　biological　samples　was　described　in　this　paper　based　on　magnetic　beads　(MBs)-based　rolling　circle　amplification　combined　with　gold　nano-particles　(AuNPs)-aptamer　labeling　technique　and　inductively　coupled　plasma　mass　spectrometry　(ICP-MS)　detection.　The　proposed　assay　has　an　ultra-high　sensitivity　and　stability,　excellent　specificity　and　more　robust　resistibility　to　the　complex　matrix　due　to　the　utilization　of　MBs-based　rolling　circle　amplification,　AuNPs-aptamer　labeling　technique　and　ICP-MS　detection.　It　can　be　used　to　determine　DNA　or　single　nucleotide　polymorphisms　(SNP)　with　an　extremely　low　detection　limit　of　0.1fM　(1.0×10-16M)　and　a　discrimination　factor　for　single-base　mismatch　of　27.　The　proposed　assay　has　been　successfully　used　to　detect　DNA　in　serum　sample　with　a　recovery　of　91-106%　and　a　relative　standard　deviation　(RSD)　

Keyword：

Diagnosis DNA Fiber optic sensors Gold nanoparticles Inductively coupled plasma Inductively coupled plasma mass spectrometry Mass spectrometers Metal nanoparticles Nanomagnetics

Community：

[ 1 ] [He, Ye]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 2 ] [Chen, Danlong]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 3 ] [Li, Mengxue]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 4 ] [Fang, Ling]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 5 ] [Yang, WeiJuan]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 6 ] [Xu, LiangJun]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 7 ] [Fu, FengFu]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China

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fengfu@fzu.edu.cn

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Source ：

Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2014

Volume： 58

Page： 209-213

6 . 4 0 9

JCR@2014

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：268

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 37

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 0

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