DNA binding in combination with capillary electrophoresis and inductively coupled plasma mass spectrometry for the rapid speciation analysis of mercury - Details

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Abstract：

We　herein　developed　a　method　for　the　rapid　speciation　analysis　of　Hg2+　and　CH3Hg+　by　using　specific　DNA　sequences　to　bind　Hg2+　and　CH3Hg+　together　with　capillary　electrophoresis　and　inductively　coupled　plasma　mass　spectrometry.　The　specific　DNA　sequences　can　combine　with　Hg2+　and　CH3Hg+　with　different　binding　force,　which　makes　the　formed　DNA-Hg2+　and　DNA-CH3Hg+　conjugates　possess　different　negative　charges,　and　thus　make　Hg2+　and　CH3Hg+　can　be　more　easily　and　rapidly　separated　by　capillary　electrophoresis　under　reverse　mode.　Under　the　optimal　conditions,　the　Hg2+　and　CH3Hg+　can　be　baseline　separated　and　detected　by　capillary　electrophoresis-inductively　coupled　plasma　mass　spectrometry　within　11 min　with　a　detection　limit　of　0.12　and　0.10 ng/mL,　respectively.　With　the　help　of　the　method,　we　have　successfully　determined　Hg2+　and　CH3Hg+　in　dried　fish　muscle　samples　with　a　recovery　of　95–101%　and　a　relative　standard　deviation　(n　=　6)　2+　and　CH3Hg+　in　the　environmental　and　food　samples.　©　2017　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim

Keyword：

Capillary electrophoresis DNA DNA sequences Inductively coupled plasma Inductively coupled plasma mass spectrometry Mercury (metal) Separation

Community：

[ 1 ] [Chen, Yiquan]Key Laboratory for Analytical Science of Food Safety and Biology of MOE, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; Fujian, China
[ 2 ] [Chen, Lian]Key Laboratory for Analytical Science of Food Safety and Biology of MOE, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; Fujian, China
[ 3 ] [Wang, Xusheng]Key Laboratory for Analytical Science of Food Safety and Biology of MOE, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; Fujian, China
[ 4 ] [Xi, Zhiming]Key Laboratory for Analytical Science of Food Safety and Biology of MOE, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; Fujian, China
[ 5 ] [Wu, Yongning]China National Center for Food Safety Risk Assessment, Beijing, China
[ 6 ] [Fu, FengFu]Key Laboratory for Analytical Science of Food Safety and Biology of MOE, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou; Fujian, China

Reprint 's Address：

付凤富
[fu, fengfu]key laboratory for analytical science of food safety and biology of moe, fujian provincial key lab of analysis and detection for food safety, college of chemistry, fuzhou university, fuzhou; fujian, china

Email：

fengfu@fzu.edu.cn

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Related Keywords：

SPECIATION OF CHROMIUM IN CAPSULES BY CAPILLARY ELECTROPHORESIS-INDUCTIVELY COUPLED PLASMA-MASS SPECTROMETRY
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2015，ELECTROPHORESIS
Speciation analysis of mercury in natural water and fish samples by using capillary electrophoresis-inductively coupled plasma mass spectrometry
2012，TALANTA

Source ：

Separation Science Plus

ISSN： 2573-1815

Year： 2018

Issue： 1

Volume： 1

Page： 52-58

1 . 3 0 0

JCR@2023

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 2

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 0

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化学学院本学院/部未明确归属的数据

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