In　this　study,　a　molybdenum　disulfide　(MoS2)　nano-sheets-modified　glassy　carbon　electrode　(GCE)　was　prepared,　and　the　electrochemical　behavior　of　baicalin　on　the　proposed　electrode　was　investigated　by　cyclic　voltammetry　(CV)　in　PBS　buffer　solution　(pH 7.0).　The　experimental　results　showed　that　baicalin　exhibits　enhanced　voltammetric　response　on　the　MoS2-modified　GCE.　Based　on　the　enhanced　voltammetric　response　of　baicalin　on　the　MoS2-modified　GCE,　a　sensitive　and　selective　electrochemical　method　for　the　determination　of　baicalin　was　developed　by　using　differential　pulse　voltammetry　(DPV).　Various　experimental　parameters　including　the　pH　of　supporting　electrolyte,　amount　of　MoS2　nano-sheets　and　accumulation　time　were　optimized.　Under　the　optimized　conditions,　the　oxidation　peak　current　was　linearly　proportional　to　the　baicalin　concentrations　in　the　range　of　1.25 × 10− 7 M–1.25 × 10− 5 M,　and　the　detection　limit　(S/N = 3)　was　calculated　to　be　5.0 × 10− 8 M.　The　developed　method　was　successfully　used　to　detect　baicalin　in　pharmaceutical　samples　with　a　recovery　of　97–109%　and　a　relative　standard　deviation　(RSD,　n = 6) 　©　2016　Elsevier　B.V.