In　order　to　investigate　the　actual　vibration　control　effect　of　the　isolated　sub-structure　for　the　mega　frame　structure,　numerical　simulation　and　shaking　table　test　of　the　(non-isolated)　mega　frame　structure　and　the　isolated　mega　frame　structure　with　isolated　sub-structure　were　performed.　The　prototype　structure　was　introduced,　then　numerical　analyses　of　the　structures　were　carried　out　using　finite　element　method,　to　study　the　seismic　response　control　effect　of　the　sub-structure　isolation　technique　towards　the　main　structure　and　the　sub-structure.　The　model　structure　and　its　test　scheme　were　introduced.　Shaking　table　tests　of　the　model　structures　were　performed,　the　seismic　responses　of　the　main　structure　and　the　sub-structure　were　measured　and　analyzed.　Analysis　and　test　results　indicate　that　the　basic　period　of　the　mega　frame　structure　with　isolated　sub-structure　is　prolonged.　The　first　six　modes　of　vibration　of　the　mega　frame　structure　with　isolated　sub-structure　are　mainly　the　sub-structure＇s　vibration,　while　the　first　six　modes　of　vibration　of　the　non-isolated　mega　frame　structure　are　mainly　the　main　structure＇s　vibration.　With　the　sub-structure　being　isolated,　the　seismic　responses　of　the　main　frame　structure　and　the　sub-structure　are　reduced　evidently.　The　seismic　responses　and　control　effects　of　the　lower　second　sub-structure　are　larger　than　the　third　sub-structure.　Lateral　deformations　of　the　isolated　sub-structures　are　concentrated　on　the　isolation　layers,　but　the　displacement　of　the　isolation　layers　are　less　than　the　width　of　the　isolation　joint.　©　2017,　Editorial　Office　of　Journal　of　Building　Structures.　All　right　reserved.