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author：

Zhao, Mengmeng (Zhao, Mengmeng.) [1] | Wang, Peilong (Wang, Peilong.) [2] | Guo, Yajuan (Guo, Yajuan.) [3] | Wang, Lixu (Wang, Lixu.) [4] | Luo, Fang (Luo, Fang.) [5] | Qiu, Bin (Qiu, Bin.) [6] | Guo, Longhua (Guo, Longhua.) [7] | Su, Xiaoou (Su, Xiaoou.) [8] | Lin, Zhenyu (Lin, Zhenyu.) [9] | Chen, Guonan (Chen, Guonan.) [10]

Indexed by：

EI

Abstract：

Aflatoxin　B1　(AFB1)　can　cause　great　threat　to　human　health,　so　the　development　of　convenient　and　portable　device　for　sensitive　detection　of　AFB1　is　highly　desired.　The　portable　pH　meter　has　the　characters　of　facile　operation,　low　cost,　and　easy　availability.　Therefore,　in　this　study,　we　investigate　the　applicability　of　utilizing　a　pH　meter　as　the　readout　to　develop　a　portable　sensor　for　AFB1.　The　specific　detection　of　AFB1　is　realized　via　the　combination　of　AFB1-responsive　aptamer-cross-linked　hydrogel.　Upon　the　addition　of　AFB1,　AFB1　binds　to　its　aptamer　with　high　affinity　in　lieu　of　aptamer/DNA　complex,　causing　the　collapse　of　hydrogel　network　and　results　in　the　releasing　of　urease　into　the　solution.　The　released　urease　can　catalyse　the　hydrolysis　of　urea　and　result　in　the　rise　of　pH　value.　The　change　of　pH　value　has　a　direct　relationship　to　the　concentration　of　AFB1　in　the　range　of　0.2–20 µM　with　a　detection　limit　of　0.1 µM　(S/N　=　3).　The　proposed　portable　device　is　successfully　applied　to　assay　AFB1　in　the　food　samples　with　satisfied　results.　©　2017　Elsevier　B.V.

Keyword：

Aflatoxins Health risks Hydrogels pH pH meters Portable equipment Urea

Community：

  • [ 1 ] [Zhao, Mengmeng]Institute of Quality Standards&Testing Technology for Agriculture Products, China Agricultural Academy of Sciences, Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Beijing; 100081, China
  • [ 2 ] [Zhao, Mengmeng]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 3 ] [Wang, Peilong]Institute of Quality Standards&Testing Technology for Agriculture Products, China Agricultural Academy of Sciences, Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Beijing; 100081, China
  • [ 4 ] [Guo, Yajuan]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 5 ] [Wang, Lixu]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 6 ] [Luo, Fang]College of Biological Science and Technology, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 7 ] [Qiu, Bin]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 8 ] [Guo, Longhua]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 9 ] [Su, Xiaoou]Institute of Quality Standards&Testing Technology for Agriculture Products, China Agricultural Academy of Sciences, Key Laboratory of Agrifood Safety and Quality, Ministry of Agriculture, Beijing; 100081, China
  • [ 10 ] [Lin, Zhenyu]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China
  • [ 11 ] [Chen, Guonan]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou; Fujian; 350116, China

Reprint 's Address：

  • [guo, longhua]moe key laboratory of analysis and detection for food safety, fujian provincial key laboratory of analysis and detection technology for food safety, department of chemistry, fuzhou university, fuzhou; fujian; 350116, china

Email：


guolh@fzu.edu.cn
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Source ：

Talanta

ISSN： 0039-9140

Year： 2018

Volume： 176

Page： 34-39

4 . 9 1 6

JCR@2018

5 . 6 0 0

JCR@2023

ESI HC Threshold：209

JCR Journal Grade：1

CAS Journal Grade：2

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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