The　electrochemiluminescent　(ECL)　behavior　of　lucigenin　on　a　multi-wall　carbon　nanotube/nano-Au　modified　glassy　carbon　electrode　(MWNT/nano-Au/GCE)　was　studied　in　this　paper.　Compared　with　the　bare　GCE,　the　ECL　intensity　of　lucigenin　can　be　greatly　enhanced　at　MWNT/nano-Au/GCE.　Based　on　the　fact　that　superoxide　dimutase　(SOD)　could　obviously　inhibit　the　ECL　of　lucigenin　at　MWNT/nano-Au/　GCE,　a　sensitive　ECL　biosensor　for　determination　of　SOD　was　developed　with　a　wide　linear　range　of　5.0　x　10(-8)-5.0　x　10(-6)　mol/L　with　detection　limit　of　2.5　x　10(-8)　mol/L　(C)　2008　Elsevier　B.V.　All　rights　reserved.