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Abstract:
A novel g-C3N4 nanosheets embedded with C3N4 QDs nanocomposites (QD@CNNS) was prepared by simple oxidation using hydrogen peroxide and UV light irradiation. This nanocomposite exhibits more stable and stronger electrochemiluminescent (ECL) behavior compared with CNNS. Coupling this nanocomposite with Fc-labeled aptamer, a signal-on aptasensor for platelet derived growth factor BB (PDGF-BB) is fabricated. Initially, the Fe-labeled aptamer binds onto QD@CNNS via pi-pi conjugation and electrostatic interaction, quenching ECL emission from QD@CNNS. The introduction of target efficiently recovers the ECL signal by the formation of PDGF-BB/aptamer complex. The ECL intensity is proportion to the concentration of PDGF-BB in the range of 0.02-80 nM with a detection limit of 0.013 nM. This work demonstrates a simple synthesis method to obtain QD@CNNS with excellent ECL behavior, and opens up the application of g-C3N4 nanocomposite in signal-on aptasensing.
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BIOSENSORS & BIOELECTRONICS
ISSN: 0956-5663
Year: 2017
Volume: 92
Page: 695-701
8 . 1 7 3
JCR@2017
1 0 . 7 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
ESI HC Threshold:226
JCR Journal Grade:1
CAS Journal Grade:1
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 1