In　this　paper,　a　novel　micro-electrochemiluminescent　(mu-ECL)　cell　with　volume　less　than　100　mu　l　was　designed　and　fabricated.　A　special　configuration　of　the　three-electrode　system,　in　which　the　counter　and　reference　electrodes　were　integrated　on　a　Perspex　optical　window　while　the　working　electrode　was　placed　opposite　to　the　optical　window,　has　greatly　decreased　the　amount　of　reagents　required　for　ECL　measurements.　In　the　mu-ECL　cell,　large　size　working　electrode　was　still　applicable,　which　maintained　ECL　detect　sensitivity　while　consuming　a　small　amount　of　sample.　In　addition,　the　immobilization　of　the　working　electrode　by　a　special　adjusting　device　facilitated　not　only　the　detachment　of　the　working　electrode　for　treatment　but　also　accurate　orientation　of　the　electrode,　consequently　improved　ECL　reproducibility,　Finally,　ECL　of　luminol　in　ionic　liquid　1-butyl-3-methylimidazolium　hexafluorophosphate　was　used　to　evaluate　this　mu-ECL　cell.　(C)　2008　Elsevier　B.V.　All　rights　reserved.