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学者姓名：张兰
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Abstract ：
Cytokinin (CK) is a type of hormone that exists widely in plants and plays a significant role in promoting cell proliferation and division. Developing a rapid and sensitive method for the detection of trace CKs remains challenging. Microporous organic networks (MONs) are novel materials widely used in sample preparation owing to their excellent extraction performance. Therefore, a dispersive solid phase extraction method based on carboxylated microporous organic networks (MON-2COOH), combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the detection of four CKs was developed. Upon introducing a carboxyl group into pure MON, the MON-2COOH material had excellent stability, high porosity, and a good dispersion effect, and provided more interaction sites to achieve good adsorption of target compounds. Under the best conditions, the established dSPE-UPLC-MS/MS method had the advantages of low adsorbent dosage (2 mg), low detection limit (1.0-10.0 pg mL-1) and good reproducibility (RSD <= 6.54%, n = 5). Synergistic extraction mechanisms involving pi-pi, hydrogen bonding and hydrophobic interactions were elucidated using both density functional theory calculations and experimental data. This work also confirmed the feasibility of functionalized MONs as satisfactory adsorbents for the enrichment of small organic compounds.
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| GB/T 7714 | Chen, Li , Yu, Tao , Huang, Le et al. Effective extraction of trace cytokinins in plants based on a carboxyl functionalized microporous organic network [J]. | ANALYTICAL METHODS , 2025 , 17 (16) : 3320-3330 . |
| MLA | Chen, Li et al. "Effective extraction of trace cytokinins in plants based on a carboxyl functionalized microporous organic network" . | ANALYTICAL METHODS 17 . 16 (2025) : 3320-3330 . |
| APA | Chen, Li , Yu, Tao , Huang, Le , Lu, Qiaomei , Zhang, Wenmin , Zhang, Lan . Effective extraction of trace cytokinins in plants based on a carboxyl functionalized microporous organic network . | ANALYTICAL METHODS , 2025 , 17 (16) , 3320-3330 . |
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Monitoring trace oxygenated polycyclic aromatic hydrocarbons (O-PAHs) in tea infusions is crucial, as long-term consumption of O-PAHs-contaminated tea can harm health. In this work, covalent organic framework (COF) TpBD-(OH)2 coating was fabricated for solid-phase microextraction (SPME) of O-PAHs. The coating contains abundant benzene rings and hydrogen bonding sites (oxygen and nitrogen groups). Density functional theory calculations, electrostatic potential distribution and X-ray photoelectron spectroscopy analysis indicated the COF efficiently adsorbs O-PAHs via van der Waals force, hydrogen bonding, electrostatic force, size matching, and π-π interaction. Under the best SPME conditions, a method for detecting O-PAHs in tea infusions was developed using gas chromatography-tandem mass spectrometry (GC–MS/MS). The method possessed low limit of detection (0.5–1.0 ng L−1), wide linearity (2.0–1000.0 ng L−1) and satisfactory recoveries (82.5 %–117.5 %). Furthermore, the method was used to study the impacts of different roasting methods on O-PAHs contamination, and results showed carbon roasting posed a higher risk. © 2025 Elsevier Ltd
Keyword ：
Covalent organic frameworks coating Covalent organic frameworks coating Gas chromatography-tandem mass spectrometry Gas chromatography-tandem mass spectrometry Oxygenated polycyclic aromatic hydrocarbons Oxygenated polycyclic aromatic hydrocarbons Tea infusion Tea infusion
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| GB/T 7714 | Yu, Q. , Li, Y. , Zhang, W. et al. Covalent organic framework coating with abundant hydrogen bonding sites for efficient enrichment and sensitive detection of oxygenated polycyclic aromatic hydrocarbons in tea [J]. | Food Chemistry , 2025 , 482 . |
| MLA | Yu, Q. et al. "Covalent organic framework coating with abundant hydrogen bonding sites for efficient enrichment and sensitive detection of oxygenated polycyclic aromatic hydrocarbons in tea" . | Food Chemistry 482 (2025) . |
| APA | Yu, Q. , Li, Y. , Zhang, W. , Cui, X. , Xie, S. , Zhang, L. . Covalent organic framework coating with abundant hydrogen bonding sites for efficient enrichment and sensitive detection of oxygenated polycyclic aromatic hydrocarbons in tea . | Food Chemistry , 2025 , 482 . |
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Background: Boronate affinity chromatography represents a poerful analytical technique for the selective separation and enrichment of biomolecules containing cis-diol moieties, including carbohydrates, glycoproteins, and other cis-dihydroxy compounds. hile boronate affinity materials (BAMs) have shon promise in glycosylation-based separation and analysis, their practical application is hindered by non-biocompatible binding pH, lo enrichment efficiency for lo-abundance samples, non-specific adsorption, and limited loading capacity. To address these limitations, this ork focuses on developing floer-like hierarchical porous diboronate affinity materials (FHP-DBAMs) ith enhanced binding strength, selectivity, and capacity for cis-diol-containing biomolecules. Results: FHP-DBAM as synthesized via a facile sol-gel method, using tetrahydroxydiboron as a hydrophilic diboronic acid monomer. The electron-ithdraing nature and hydrophilicity of diboronate affinity mechanism enable FHP-DBAM to operate at loer pH values (pH ≥ 5), addressing the biocompatibility issue. DFT and experiment calculations confirm the enhanced cis-diol binding affinity of diboronate affinity mechanism compared ith monoboronate affinity mechanism, resulting in a remarkably lo dissociation constant (DFT Kd = 6.74 × 10−5 M, experiment Kd = 9.95 × 10−5 M) for FHP-DBAM. Furthermore, the unique floer-like hierarchical porous structure provides a high surface area and nanoconfinement effect, significantly boosting target molecule loading capacity and affinity reaction kinetics. Compared to traditional BAMs, FHP-DBAM exhibits over ten times higher loading capacity. As a proof-of-concept, FHP-DBAM successfully captures the biomarker GM1 in breast cancer cells MCF-7 ith high efficiency. Significance and novelty: This ork introduces diboronate affinity mechanism and floer-like hierarchical porous structure as ne solution to overcome the limitations of conventional BAMs. FHP-DBAMs achieve loer binding pH, enhanced selectivity, and stronger binding stability through diboronate affinity mechanism. The unique floer-like porous structure maximizes surface area and active sites, addressing lo enrichment efficiency and loading capacity. These advancements are critical for the efficient and biocompatible separation of cis-diol-containing biomolecules. © 2025 Elsevier B.V.
Keyword ：
Biomarker Biomarker Controllable synthesis Controllable synthesis Diboronate affinity mechanism Diboronate affinity mechanism Floer-like hierarchical porous Floer-like hierarchical porous
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| GB/T 7714 | Xu, J. , Tang, M. , Zhang, E. et al. Controlled synthesis of superhydrophilic floer-like hierarchical porous diboronate affinity materials for capturing biomarkers [J]. | Analytica Chimica Acta , 2025 , 1357 . |
| MLA | Xu, J. et al. "Controlled synthesis of superhydrophilic floer-like hierarchical porous diboronate affinity materials for capturing biomarkers" . | Analytica Chimica Acta 1357 (2025) . |
| APA | Xu, J. , Tang, M. , Zhang, E. , Xie, S. , Gu, Q. , Zhang, L. . Controlled synthesis of superhydrophilic floer-like hierarchical porous diboronate affinity materials for capturing biomarkers . | Analytica Chimica Acta , 2025 , 1357 . |
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Covalent organic framework (COF) film-based solid-phase extraction (F-SPE) has garnered great attention in sample pretreatment. However, harsh synthesis conditions of COF films have severely hindered their potential applications. In this study, a kind of COF (TPB-DMTP) films were fabricated via a liquid-liquid interfacial synthesis method at a mild condition. The obtained films exhibited excellent extraction performance towards microcystins (MCs, an algal toxin) due to their porous structure, high specific surface area and abundant accessible adsorption sites. Coupled TPB-DMTP films-based F-SPE with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), a sensitive and environment-friendly analytical method was established for MCs detection. Under the optimal conditions, this method possessed wide linear ranges (2.0-800.0 pg mL-1) with good linearity (R >= 0.9991), low limits of detection (0.8-3.0 pg mL-1) and satisfactory precision (RSDs <= 7.1 %), which then successfully applied for MCs detection in actual aquatic organism samples. Trace amounts of MC-RR (42.4 pg mL-1) and MC-YR (14.6 pg mL-1) were detected in the mussels. The results demonstrate the excellent application potential of COF films in sample pretreatment.
Keyword ：
Covalent organic frameworks Covalent organic frameworks Films Films HPLC-MS/MS HPLC-MS/MS Interface-assisted synthesis Interface-assisted synthesis Microcystins Microcystins
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| GB/T 7714 | Zhang, Wenmin , Wang, Zhiyong , Zhang, Lan . Interface-assisted synthesized covalent organic framework film for efficient extraction of microcystins in aquatic organisms [J]. | TALANTA , 2025 , 282 . |
| MLA | Zhang, Wenmin et al. "Interface-assisted synthesized covalent organic framework film for efficient extraction of microcystins in aquatic organisms" . | TALANTA 282 (2025) . |
| APA | Zhang, Wenmin , Wang, Zhiyong , Zhang, Lan . Interface-assisted synthesized covalent organic framework film for efficient extraction of microcystins in aquatic organisms . | TALANTA , 2025 , 282 . |
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Adsorbents with good dispersibility and high efficiency are crucial for magnetic solid-phase extraction (MSPE). In this study, flower-like magnetic nanomaterials (F-Ni@NiO@ZnO2-C) were successfully prepared by calcination of metal-organic framework (MOF) precursors that was stacked by two-dimensional (2D) nanosheet. The synthesized F-Ni@NiO@ZnO2-C has a flower-like layered structure with a large amount of pore space, promoting the rapid diffusion of targets. In addition, Zn2+ doped in MOF precursors was still retained that further produced strong metal chelation with targets. The unique structure of F-Ni@NiO@ZnO2-C was used as MSPE adsorbent, and combined with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for extraction of three microcystins (MCs) detection, including microcystin-LR (MC-LR), microcystin-RR (MC-RR), microcystin-YR (MC-YR). The resulting method has a detection limit of 0.2–1.0 pg mL−1, a linear dynamic range of 0.6–500.0 pg mL−1 and has good linearity (R ≥ 0.9996). Finally, the established method was applied to the highly selective enrichment of MCs in biological samples, successfully detecting trace amounts of MCs (8.4–15.0 pg mL−1) with satisfactory recovery rates (83.7–103.1 %). The results indicated that flower-like magnetic F-Ni@NiO@ZnO2-C was a promising adsorbent, providing great potential for the determination of trace amounts of MCs in biological samples. © 2024 Elsevier B.V.
Keyword ：
Carbon Carbon Extraction Extraction High performance liquid chromatography High performance liquid chromatography Mass spectrometry Mass spectrometry Nanosheets Nanosheets Nickel oxide Nickel oxide Organometallics Organometallics Phase separation Phase separation
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| GB/T 7714 | Xie, Shiye , Liao, Baodi , Yu, Jing et al. Self-assembled flower-like carbon nanosheets for magnetic solid-phase extraction of microcystins from aquatic organism [J]. | Journal of Chromatography A , 2024 , 1730 . |
| MLA | Xie, Shiye et al. "Self-assembled flower-like carbon nanosheets for magnetic solid-phase extraction of microcystins from aquatic organism" . | Journal of Chromatography A 1730 (2024) . |
| APA | Xie, Shiye , Liao, Baodi , Yu, Jing , Zhang, Wenmin , Chen, Hui , Xu, Jinhua et al. Self-assembled flower-like carbon nanosheets for magnetic solid-phase extraction of microcystins from aquatic organism . | Journal of Chromatography A , 2024 , 1730 . |
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Membrane-based micro-solid phase extraction (M-μSPE) has garnered great attention in sample pretreatment, suffering an inherent contradiction between permeability and adsorption capacity. In this study, a pure microporous organic network (TEB-DIB-MON) fiber membrane was prepared by combining electrostatic spinning technology, Sonogashira-Hagihara reaction and template sacrifice method. The prepared TEB-DIB-MON membrane exhibited a large specific surface area with a hollow and porous structure, thereby providing excellent solvent permeability and high adsorption capacity for okadaic acid (OA, an algal toxin). Under the optimized conditions, a sensitive analytical method was established by coupling M-μSPE with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The established method has a low detection limit (0.5 pg mL−1), a wide linear range (1.5–1000 pg mL−1, R ≥ 0.9991), and good reproducibility (RSD ≤ 9.4 %, n = 6), which was then successfully applied for OA detection in marine organisms. Trace amounts of OA (59.3–89.0 pg mL−1) was detected in the oyster and prawn samples. This work demonstrated that the excellent application potential of MON membranes in sample pretreatment, while also presents a novel synthesis strategy for MONs membranes. © 2024 Elsevier B.V.
Keyword ：
Fibrous membranes Fibrous membranes Micropores Micropores Nafion membranes Nafion membranes Spinning (fibers) Spinning (fibers)
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| GB/T 7714 | Zhang, Wenmin , Liao, Baodi , Xie, Shiye et al. Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms [J]. | Journal of Chromatography A , 2024 , 1736 . |
| MLA | Zhang, Wenmin et al. "Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms" . | Journal of Chromatography A 1736 (2024) . |
| APA | Zhang, Wenmin , Liao, Baodi , Xie, Shiye , Zhang, Lan . Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms . | Journal of Chromatography A , 2024 , 1736 . |
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Red tides are a type of natural marine disaster caused by harmful algae characterized by a high toxicity, wide distribution, and long duration. Since the concentration of algal toxins in seawater increases with the occurrence of red tides, algal toxins detected in seawater could be used to predict the occurrence and evolution of red tides. Brevetoxin-A (BTX-A) is a secondary metabolite produced by the harmful algae Karenia brevis, whose detection in seawater could form the basis of an accurate warning system for incoming red tides. However, due to the inherent complexity of the seawater matrix and the extremely low levels of BTX-A in seawater, the use of instruments for its direct detection is difficult. Therefore, there is an urgent need to develop a sample pretreatment method for the efficient enrichment of BTX-A in seawater. In this study, a metal-organic backbone material (UiO-66) and its composite with silica microspheres (SiO2@UiO-66) were successfully synthesized using the solvothermal method. The prepared SiO2@UiO-66 exhibited good hydrophilicity, water stability, and large specific surface area. Furthermore, it also exhibited hydrogen bonding and electrostatic interactions with BTX-A, had a strong affinity for BTX-A, and was able to efficiently adsorb BTX-A in complex matrices. Therefore, SiO2@UiO-66 showed potential as a novel packing material for the extraction of BTX-A from solid phase extraction columns. Combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), a highly sensitive detection method for the determination of BTX-A in marine water was established. The established analytical method had a low detection limit (3.0 pg/mL), a wide linear range (10.0 -200.0 pg/mL), and a good linear relationship (R=0.9992). Combined with the Fujian Province Red Tide Monitoring and Early Warning Information 2021 issued by the Fujian Provincial Oceanic and Fisheries Bureau, the analytical method established herein was successfully applied to analyze and monitor the content of BTX-A in actual seawater samples. This highlights the proposed system's potential for use as an early warning factor in the monitoring of red tides, representing a simple and fast pretreatment methodology for the detection of BTX-A in seawater.
Keyword ：
brevetoxin-A brevetoxin-A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) metal-organic frameworks (MOFs) metal-organic frameworks (MOFs) red tide red tide solid phase extraction (SPE) solid phase extraction (SPE)
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| GB/T 7714 | Chen, Z.-B. , Xie, S.-Y. , Liu, Y.-J. et al. Zirconium-based metal-organic framework composites for solid phase extraction of brevetoxin-A from seawater [J]. | Se pu = Chinese journal of chromatography , 2024 , 42 (9) : 819-826 . |
| MLA | Chen, Z.-B. et al. "Zirconium-based metal-organic framework composites for solid phase extraction of brevetoxin-A from seawater" . | Se pu = Chinese journal of chromatography 42 . 9 (2024) : 819-826 . |
| APA | Chen, Z.-B. , Xie, S.-Y. , Liu, Y.-J. , Zhang, W.-M. , Fang, M. , Zhang, L. . Zirconium-based metal-organic framework composites for solid phase extraction of brevetoxin-A from seawater . | Se pu = Chinese journal of chromatography , 2024 , 42 (9) , 819-826 . |
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短裸甲藻毒素(BTX-A)是由有害藻类短裸甲藻产生的次生代谢物,检测海水中的藻毒素可以预测赤潮的发生和增长.将BTX-A含量监测作为赤潮预警的因子之一可以有效提高赤潮预警的准确性,开发一种高效富集海水中BTX-A的样品前处理方法迫在眉睫.研究通过溶剂热法制备了具有较大比表面积(310.9 m2/g)、对BTX-A有较强吸附作用的微米级Zr-MOFs复合材料(SiO2@UiO-66),并将其作为固相萃取(SPE)填料,结合高效液相色谱-串联质谱(HPLC-MS/MS)技术,建立了一种检出限低(3.0 pg/mL)、线性范围宽(10.0～200.0 pg/mL)、重复性良好(RSD≤8.5%,n=6)的海水中 BTX-A的高灵敏检测方法.所建立的分析方法成功用于实际海水样品中BTX-A含量的分析和监测,结合福建省海洋与渔业局所发布的赤潮监测预警信息,可作为赤潮预警因子有效提高赤潮的监测和预警.
Keyword ：
固相萃取 固相萃取 短裸甲藻毒素 短裸甲藻毒素 赤潮 赤潮 金属有机骨架 金属有机骨架 高效液相色谱-串联质谱 高效液相色谱-串联质谱
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| GB/T 7714 | 陈宗保 , 谢诗烨 , 刘泳君 et al. 锆基金属有机骨架复合材料用于海水中短裸甲藻毒素的固相萃取 [J]. | 色谱 , 2024 , 42 (9) : 819-826 . |
| MLA | 陈宗保 et al. "锆基金属有机骨架复合材料用于海水中短裸甲藻毒素的固相萃取" . | 色谱 42 . 9 (2024) : 819-826 . |
| APA | 陈宗保 , 谢诗烨 , 刘泳君 , 张文敏 , 方敏 , 张兰 . 锆基金属有机骨架复合材料用于海水中短裸甲藻毒素的固相萃取 . | 色谱 , 2024 , 42 (9) , 819-826 . |
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Background: Accurate quantitative analysis of small molecule metabolites in biological samples is of great significance. Hydroxypolycyclic aromatic hydrocarbons (OH-PAHs) are metabolic derivatives of emerging pollutants, reflecting exposure to polycyclic aromatic hydrocarbons (PAHs). Macromolecules such as proteins and enzymes in biological samples will interfere with the accurate quantification of OH-PAHs, making direct analysis impossible, requiring a series of complex treatments such as enzymatic hydrolysis. Therefore, the development of matrix-compatible fiber coatings that can exclude macromolecules is of great significance to improve the ability of solid-phase microextraction (SPME) technology to selectively quantify small molecules in complex matrices and achieve rapid and direct analysis. Results: We have developed an innovative coating with a stable macromolecular barrier using electrospinning and flexible filament winding (FW) technologies. This coating, referred to as the hollow fibrous covalent organic framework@polyionic liquid (F-COF@polyILs), demonstrates outstanding conductivity and stability. It accelerates the adsorption equilibrium time (25 min) for polar OH-PAHs through electrically enhanced solid -phase microextraction (EE-SPME) technology. Compared to the powder form, F-COF@polyILs coating displays effective non -selective large -size molecular sieving. Combining gas chromatography -tandem triple quadrupole mass spectrometry (GC-MS/MS), we have established a simple, efficient quantitative analysis method for OH-PAHs with a low detection limit (0.008-0.05 ng L-1), wide linear range (0.02-1000 ng L-1), and good repeatability (1.0%-7.3 %). Experimental results show that the coated fiber exhibits good resistance to matrix interference (2.5%-16.7 %) in complex biological matrices, and has been successfully used for OH-PAHs analysis in human urine and plasma. Significance: FW technology realizes the transformation of the traditional powder form of COF in SPME coating to a uniform non -powder coating, giving its ability to exclude large molecules in complex biological matrices. A method for quantitatively detecting OH-PAHs in real biological samples was also developed. Therefore, the filament winding preparation method for F-COF@polyILs coated fibers, along with fibrous COFs' morphology control, has substantial implications for efficiently extracting target compounds from complex matrices.
Keyword ：
Covalent organic frameworks Covalent organic frameworks Filament winding Filament winding GC-MS GC-MS Monohydroxy polycyclic aromatic hydrocarbons Monohydroxy polycyclic aromatic hydrocarbons Solid phase microextraction Solid phase microextraction
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| GB/T 7714 | Wang, Jingyi , Zhang, Wenmin , Ding, Qingqing et al. Flexible filament winding strategy to prepare COF@polyionic liquid-coated fibers for non-selective exclusion of macromolecules in electro-enhanced solid-phase microextraction [J]. | ANALYTICA CHIMICA ACTA , 2024 , 1306 . |
| MLA | Wang, Jingyi et al. "Flexible filament winding strategy to prepare COF@polyionic liquid-coated fibers for non-selective exclusion of macromolecules in electro-enhanced solid-phase microextraction" . | ANALYTICA CHIMICA ACTA 1306 (2024) . |
| APA | Wang, Jingyi , Zhang, Wenmin , Ding, Qingqing , Xu, Jinhua , Yu, Qidong , Zhang, Lan . Flexible filament winding strategy to prepare COF@polyionic liquid-coated fibers for non-selective exclusion of macromolecules in electro-enhanced solid-phase microextraction . | ANALYTICA CHIMICA ACTA , 2024 , 1306 . |
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Membrane-based micro-solid phase extraction (M-mu SPE) has garnered great attention in sample pretreatment, suffering an inherent contradiction between permeability and adsorption capacity. In this study, a pure micro- porous organic network (TEB-DIB-MON) fiber membrane was prepared by combining electrostatic spinning technology, Sonogashira-Hagihara reaction and template sacrifice method. The prepared TEB-DIB-MON membrane exhibited a large specific surface area with a hollow and porous structure, thereby providing excellent solvent permeability and high adsorption capacity for okadaic acid (OA, an algal toxin). Under the optimized conditions, a sensitive analytical method was established by coupling M-mu SPE with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The established method has a low detection limit (0.5 pg mL-1),- 1 ), a wide linear range (1.5-1000 pg mL-1,- 1 , R >= 0.9991), and good reproducibility (RSD <= 9.4 %, n = 6), which was then successfully applied for OA detection in marine organisms. Trace amounts of OA (59.3-89.0 pg mL-- 1) was detected in the oyster and prawn samples. This work demonstrated that the excellent application potential of MON membranes in sample pretreatment, while also presents a novel synthesis strategy for MONs membranes.
Keyword ：
Fiber membrane Fiber membrane HPLC-MS/MS HPLC-MS/MS Membrane-solid phase extraction Membrane-solid phase extraction Microporous organic networks Microporous organic networks Okadaic acid Okadaic acid
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| GB/T 7714 | Zhang, Wenmin , Liao, Baodi , Xie, Shiye et al. Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms [J]. | JOURNAL OF CHROMATOGRAPHY A , 2024 , 1736 . |
| MLA | Zhang, Wenmin et al. "Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms" . | JOURNAL OF CHROMATOGRAPHY A 1736 (2024) . |
| APA | Zhang, Wenmin , Liao, Baodi , Xie, Shiye , Zhang, Lan . Hollow microporous organic network fiber membrane for efficient extraction of okadaic acid from marine organisms . | JOURNAL OF CHROMATOGRAPHY A , 2024 , 1736 . |
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