In　this　article,　a　CE　with　a　new　electrochemiluminescent　(ECL)　detection　system　was　developed.　A　microfluidic　ECL　detection　cell　with　less　than　0.5　mu　L　dead　volumes　was　developed　and　used　as　detector　for　this　system.　A　hydrofluoric　acid-etched　porous　joint　was　made　at　8　mm　from　the　outlet　of　the　separation　capillary　to　isolate　the　CE　high　voltage　from　ECL　detection.　The　proposed　CE-ECL　system　was　applied　for　separation　and　detection　of　some　proteins　labeled　with　tris(1,10-phenanthroline)　ruthenium(II).　High　efficiency　ECL-enhanced　reagent,　tripropylamine,　was　infused　to　the　detection　cell　as　coreactant　by　a　micro-infusion　system　to　obtain　maximum　and　stable　ECL　signal.　The　performance　of　this　setup　was　illustrated　by　the　analysis　of　tris(1,10-phenanthroline)　ruthenium(II)-labeled　proteins.　The　background　electrolyte　for　protein　detection　was　20　mM　Tris-CH3COOH　with　2.0%　m/m.　PVP　at　pH　4.0.　Under　the　optimal　conditions,　the　corresponding　LOD　were　2.2　x　10(-10)　M　for　HSA,　4.4　x　10(-10)　M　for　casein　(alpha-S1)　and　5.1　x　10(-10)　M　for　cytochrome　c.　The　proposed　method　was　also　successfully　used　for　the　trace　analysis　of　albumin　in　human　urine　without　any　pretreatment.　Keywords: