In　this　study,　the　electrochemiluminescent　(ECL)　behavior　of　Nickel(II)　tetrasulfophthalocyanine　(NiTSPc)/H2O2　on　a　heated　indium　tin　oxide　(ITO)　electrode　was　investigated.　The　effect　of　pH　value,　electrochemical　scan　mode,　concentration　of　NiTSPc　and　electrode　surface　temperature　on　the　ECL　intensities　had　been　studied　in　detail.　Based　on　the　fact　that　the　ECL　of　NiTSPc　can　be　greatly　enhanced　by　hydrogen　peroxide　at　the　ITO　electrode,　a　new　ECL　biosensor　for　hydrogen　peroxide　has　been　developed.　The　possible　mechanism　for　the　ECL　of　NiTSPc　has　also　been　proposed.　(C)　2012　Zhen　Yu　Lin.　Published　by　Elsevier　B.V.　on　behalf　of　Chinese　Chemical　Society.　All　rights　reserved.