Four　novel　tetracyclic　oxindole　alkaloids,　namely,　speradines　B　(1),　C　(2),　D　(3),　and　E　(4)　were　isolated　from　the　marine-derived　fungus　Aspergillus　oryzae.　The　structures　of　these　compounds　without　the　absolute　configurations　were　elucidated　through　1D　and　2D　nuclear　magnetic　resonance　and　high-resolution　mass　spectrometric　analyses.　Among　these　compounds,　1　and　4　showed　weak　cytotoxic　effects　on　the　He　La　cell　line.