A　disposable,　equipment-free,　versatile　point-of-care　testing　platform,　microfluidic　distance　readout　sweet　hydrogel　integrated　paper-based　analytical　device　(mu　DiSH-PAD),　was　developed　for　portable　quantitative　detection　of　different　types　of　targets.　The　platform　relies　on　a　target-responsive　aptamer　cross　linked　hydrogel　for　target　recognition,　cascade　enzymatic　reactions　for　signal　amplification,　and　microfluidic　paper-based　analytic　devices　(mu　PADs)　for　visual　distance-based　quantitative　readout.　A　＂sweet＂　hydrogel　with　trapped　glucoamylase　(GA)　was　synthesized　using　an　aptamer　as　a　cross-linker.　When　target　is　present　in　the　sample,　the　＂sweet＂　hydrogel　collapses　and　releases　enzyme　GA　into　the　sample,　generating　glucose　by　amylolysis.　A　hydrophilic　channel　on　the　mu　PADs　is　modified　with　glucose　oxidase　(GOx)　and　colorless　3,3＇-diaminobenzidine　(DAB)　as　the　substrate.　When　glucose　travels　along　the　channel　by　capillary　action,　it　is　converted　to　H2O2　by　GOx.　In　addition,　DAB　is　converted　into　brown　insoluble　poly-3,3＇-diaminobenzidine　[poly(DAB)]　by　horseradish　peroxidase,　producing　a　visible　brown　bar,　whose　length　is　positively　correlated　to　the　concentration　of　targets.　The　distance-based　visual　quantitative　platform　can　detect　cocaine　in　urine　with　high　selectivity,　sensitivity,　and　accuracy.　Because　the　target-induced　cascade　reaction　is　triggered　by　aptamer/target　recognition,　this　method　is　widely　suitable　for　different　kinds　of　targets.　With　the　advantages　of　low　cost,　ease　of　operation,　general　applicability,　and　disposability　with　quantitative　readout,　the　mu　DiSH-PAD　holds　great　potential　for　portable　detection　of　trace　targets　in　environmental　monitoring,　security　inspection,　personalized　healthcare,　and　clinical　diagnostics.