A　disposable,　equipment-free,　versatile　point-of-care　testing　platform,　microfluidic　distance　readout　sweet　hydrogel　integrated　paper-based　analytical　device　(μDiSH-PAD),　was　developed　for　portable　quantitative　detection　of　different　types　of　targets.　The　platform　relies　on　a　target-responsive　aptamer　cross-linked　hydrogel　for　target　recognition,　cascade　enzymatic　reactions　for　signal　amplification,　and　microfluidic　paper-based　analytic　devices　(μPADs)　for　visual　distance-based　quantitative　readout.　A　＇sweet＇　hydrogel　with　trapped　glucoamylase　(GA)　was　synthesized　using　an　aptamer　as　a　cross-linker.　When　target　is　present　in　the　sample,　the　＇sweet＇　hydrogel　collapses　and　releases　enzyme　GA　into　the　sample,　generating　glucose　by　amylolysis.　A　hydrophilic　channel　on　the　μPADs　is　modified　with　glucose　oxidase　(GOx)　and　colorless　3,3′-diaminobenzidine　(DAB)　as　the　substrate.　When　glucose　travels　along　the　channel　by　capillary　action,　it　is　converted　to　H2O2　by　GOx.　In　addition,　DAB　is　converted　into　brown　insoluble　poly-3,3′-diaminobenzidine　[poly(DAB)]　by　horseradish　peroxidase,　producing　a　visible　brown　bar,　whose　length　is　positively　correlated　to　the　concentration　of　targets.　The　distance-based　visual　quantitative　platform　can　detect　cocaine　in　urine　with　high　selectivity,　sensitivity,　and　accuracy.　Because　the　target-induced　cascade　reaction　is　triggered　by　aptamer/target　recognition,　this　method　is　widely　suitable　for　different　kinds　of　targets.　With　the　advantages　of　low　cost,　ease　of　operation,　general　applicability,　and　disposability　with　quantitative　readout,　the　μDiSH-PAD　holds　great　potential　for　portable　detection　of　trace　targets　in　environmental　monitoring,　security　inspection,　personalized　healthcare,　and　clinical　diagnostics.　©　2016　American　Chemical　Society.