Removal　of　Pb2+　and　biodegradation　of　organophosphorus　have　been　both　widely　investigated　respectively.　However,　bio-remediation　of　both　Pb2+　and　organophosphorus　still　remains　largely　unexplored.　Bacillus　subtilis　FZUL-33,　which　was　isolated　from　the　sediment　of　a　lake,　possesses　the　capability　for　both　biomineralization　of　Pb2+　and　biodegradation　of　acephate.　In　the　present　study,　both　Pb2+　and　acephate　were　simultaneously　removed　via　biodegradation　and　biomineralization　in　aqueous　solutions.　Batch　experiments　were　conducted　to　study　the　influence　of　pH,　interaction　time　and　Pb2+　concentration　on　the　process　of　removal　of　Pb2+.　At　the　temperature　of　25`degrees　C,　the　maximum　removal　of　Pb2+　by　B.　subtilis　FZUL-33　was　381.31　11.46　mg/g　under　the　conditions　of　pH　5.5,　initial　Pb2+　concentration　of　1300　mg/L,　and　contact　time　of　10　min.　Batch　experiments　were　conducted　to　study　the　influence　of　acephate　on　removal　of　Pb2+　and　the　influence　of　Pb?*　on　biodegradation　of　acephate　by　B.　subtilis　FZUL-33.　In　the　mixed　system　of　acephate-Pb2+,　the　results　show　that　biodegradation　of　acephate　by　B.　subtilis　FZUL-33　released　POI＇,　which　promotes　mineralization　of　Pb2+.　The　process　of　biodegradation　of　acephate　was　affected　slightly　when　the　concentration　of　Pb2+　was　below　100　mg/L.　Based　on　the　results,　it　can　be　inferred　that　the　B.　subtilis　FZUL-33　plays　a　significant　role　in　bio-remediation　of　organophosphorus-heavy　metal　compound　contamination.　(C)　2016　The　Research　Center　for　Eco-Environmental　Sciences,　Chinese　Academy　of　Sciences.　Published　by　Elsevier　B.V.