Sensitive　and　accurate　test　of　blood　glucose　levels　is　necessary　to　monitor　and　prevent　diabetic　complications.　Herein,　we　developed　a　novel　and　sensitive　non-enzymatic　glucose　sensing　platform　by　employing　3D　hierarchical　porous　Au　networks　(HPANs)　as　electrocatalyst　for　glucose　oxidization.　The　HPANs　were　prepared　through　a　bio-inspired　synthesis　method,　in　which　the　natural　eggshell　membrane　(ESM)　was　introduced　as　template.　The　structure　and　properties　of　the　as-prepared　HPANs　were　characterized　by　a　set　of　techniques,　including　scanning　electron　microscope　(SEM),　energy-dispersive　X-ray　spectroscopy　(EDS),　powder　X-ray　diffraction　(XRD)　and　cyclic　voltammetry　(CV).　The　HPANs　showed　high　catalytic　activity　towards　glucose　oxidization　due　to　the　unique　structure.　Inspiringly,　the　HPANs-based　electrochemical　glucose　sensor　could　be　driven　at　low　potential　(+0.1　V)　and　showed　an　outstanding　performance　for　glucose　determination　with　two　linear　ranges　of　1-500　mu　M　and　4.0-12　mM,　a　limit　of　detection　(LOD)　of　0.2　mu　M　(3　sigma),　and　fast　response　time　(less　than　2　s).　Moreover,　the　stability　and　anti-interference　performance　of　developed　sensor　was　also　excellent,　enabling　its　preliminary　application　in　clinical　sample　(human　serum)　test.　Significantly,　this　work　offered　an　environmentally　friendly　method　for　fabricating　3D　nanostructure　by　using　ESM　(a　biowaste)　as　template,　setting　up　a　typical　example　for　producing　new　value-added　nanomaterials　with　sensing　application.