Polydiallyldimethylammonium　chloride,　PDDA,　was　used　as　a　stabilizer　and　linker　for　functionalized　TiO2　nano　tubes　(TiO2　NTs).　Self-assembled　process　with　palladium　nanoparticles　(NPs)　was　successfully　synthesized　and　used　for　the　oxidation　of　glucose　on　glassy　carbon　electrodes.　Based　on　the　voltammetric　and　amperometric　results,　Pd　NPs　efficiently　catalyzed　the　oxidation　of　glucose　at　-　0.05　V　in　the　presence　of　0.1　M　NaCl　and　showed　excellent　resistance　toward　interference　poisoning　from　such　interfering　species　as　ascorbic　acid,　uric　acid　and　urea.　To　further　increase　sensitivity,　the　Pd　NPs-PDDA-TiO2　NTs/GCE　was　electrochemically　treated　with　H2SO4　and　NaOH,　the　glucose　oxidation　current　was　magnified　2.5　times　than　that　before　pretreatments　due　to　greatly　enhancing　the　electron　transport　property　of　the　sensor　based　on　the　increased　defect　sites　and　surface　oxide　species.　In　view　of　the　physiological　level　of　glucose,　the　wide　linear　concentration　range　of　glucose　(4　x　10(-7)-8　x　10(-4)　M)　with　a　detection　limit　of　8　x　10(-8)　M　(S/N　=　3)　was　obviously　good　enough　for　clinical　application.　(C)　2016　Elsevier　B.V.　All　rights　reserved.