A　new　and　signal-on　photoelectrochemical　(PEC)　sensing　platform　was　successfully　designed　for　the　sensitive　detection　of　prostate-specific　antigen　(PSA),　using　reduced　graphene　oxide-　functionalized　iron　oxyhydroxide　(FeOOH-rGO)　as　the　photoactive　material,　accompanying　target-responsive　controlled　release　system　to　achieve　the　signal　amplification.　Introduction　of　rGO　as　electron　mediator　greatly　facilitated　the　electron　transfer　from　FeOOH　to　electrode　under　visible　light,　which　inhibited　the　electron-hole　recombination　to　enhance　the　photo-activity　of　FeOOH-rGO.　Additionally,　the　bioresponsive　release　system　was　controlled　via　the　reaction　of　target　PSA　with　the　aptamer　capped　glucose-loading　mesoporous　silica　nanoparticle　(MSN)　to　release　numerous　glucose　molecules　(as　the　electron　donors)　for　the　amplification　of　the　photocurrent　generated　from　FeOOHrGO.　Thus,　more　glucose　molecules　could　be　released　and　enhanced　photocurrents　could　be　obtained　with　the　increasing　PSA　concentrations.　Experimental　results　showed　that　the　photocurrents　of　the　PEC　sensing　platform　were　linearly　dependent　on　the　logarithm　of　PSA　concentrations　from　1.0　pg/mL　to　100　ng/mL.　Moreover,　the　PEC　sensing　system　afforded　good　stability　and　specificity,　and　its　accuracy　matched　well　with　the　commercial　PSA　enzyme-linked　immunosorbent　assay　(ELISA)　kit.　The　excellent　performance　of　the　PEC　sensing　platform　indicated　its　promising　prospect　as　a　useful　tool　for　PSA　detection　in　practical　application.