Herein　a　novel　split-type　photoelectrochemical　(PEC)　immunosensing　platform　was　designed　for　sensitive　detection　of　low-abundance　biomarkers　(prostate-specific　antigen,　PSA,　used　in　this　case)　by　coupling　a　peroxyoxalate　chemiluminescence　(PO-CL)　self-illuminated　system　with　digital　multimeter　(DMM)　readout.　The　PEC　detection　device　consisted　of　a　capacitor/DMM-joined　electronic　circuit　and　a　PO-CL-based　self-illuminated　cell.　Initially,　reduced　graphene　oxide-doped　BiVO4　(BiVO4-rGO)　photovoltaic　materials　with　good　photoelectric　properties　was　integrated　into　the　capacitor/DMM-joined　circuit　for　photocurrent　generation　in　the　presence　of　hydrogen　peroxide　(H2O2,　as　the　hole-trapping　reagent).　A　sandwich-type　immunoreaction　with　target　PSA　was　carried　out　in　capture　antibody-coated　microplates　by　using　glucose　oxidase/detection　antibody-conjugating　gold　nanoparticle　(pAb(2)-AuNP-GOx).　Accompanying　the　sandwiched　immunocomplex,　the　labeled　GOx　could　oxidize　glucose　to　produce　H2O2.　The　as-generated　H2O2　could　act　as　the　coreaction　reagent　to　trigger　the　chemiluminescence　of　the　peroxyoxalate　system　and　the　PEC　reaction　of　the　BiVO4-rGO.　Meanwhile,　the　self-illuminated　light　could　induce　photovoltaic　material　(BiVO4-rGO)　to　produce　a　voltage　that　was　utilized　to　charge　an　external　capacitor.　With　the　switch　closed,　the　capacitor　could　discharge　through　the　DMM　and　provide　an　instantaneous　current.　Different　from　conventional　PEC　immunoassays,　the　as　generated　photoelectron　was　stored　in　the　capacitor　and　released　instantaneously　to　amplify　the　photocurrent.　Under　the　optimal　conditions,　the　transient　current　increased　with　the　increasing　target　PSA　concentration　in　the　dynamic　working　range　from　10　pg　mL(-1)　to　80　ng　mL(-1)　with　a　detection　limit　(LOD)　of　3　pg　mL(-1).　This　work　demonstrated　for　the　first　time　that　the　peroxyoxalate　CL　system　could　be　used　as　a　suitable　substitute　of　physical　light　source　to　apply　in　PEC　immunoassay.　In　addition,　this　methodology　afforded　good　reproducibility,　precision,　and　high　specificity,　and　the　method　accuracy　matched　well　with　the　commercial　PSA　ELISA　kit.　Importantly,　the　developed　split-type　photoelectrochemical　immunoassay　could　not　only　avoid　the　interfering　of　the　biomolecules　relative　to　the　photovoltaic　materials　but　also　eliminate　the　need　of　an　exciting　light　source　and　expensive　instrumentation,　thus　representing　a　user-friendly　and　low-cost　assay　protocol　for　practical　utilization　in　quantitative　low-abundance　proteins.