CuI-BiOI/Cu　film　with　effective　photocatalytic　inactivation　of　E.　coli　under　visible-light-driven　(VLD)　were　prepared　on　copper　sheet　and　were　characterized　by　TEM,　SEM,　XRD,　XPS　and　DRS.　The　photocatalytic　inactivation　of　E.　coli　of　Cud,　BiOI,　CuI-BiOI　and　CuI-BiOI/Cu　film　were　investigated.　The　results　showed　that　CuIBiOI/Cu　film　exhibit　the　most　excellent　bacteria　disinfection　activity,　and　the　quantity　of　viable　bacteria　could　almost　inactivate　after　being　illuminated　for　2　h.　The　Laser　Scanning　Confocal　Microscopy　(LSCM)　observation　exhibits　that　the　morphology　of　bacteria　changed　a　lot　after　visible　light　irradiation,　and　the　UV　spectra　confirm　the　leakage　of　bacterial　protein.　The　antibacterial　mechanism　was　studied　by　employing　photoelectrochemical　techniques　and　scavengers　of　different　reactive　species,　which　reveals　that　the　effect　of　reactive　species　on　the　antibacterial　activity　decrease　in　order　h(+)　＞　e(-)　＞　H2O2　＞　center　dot　HO　＞　center　dot　O-2(-).　The　CuI　and　Cu　play　the　important　role　in　the　separation　of　h+　and　During　the　photocatalytic　process,　the　CuI　act　as　a　hole-transport　channel　and　the　Cu　act　as　electron　transfer　for　CuI-BiOI　heterostructure,　which　promotes　the　separation　of　photo　generated　charges　and　enhances　the　photocatalytic　antibacterial　activity　of　the　as-prepared　samples.