In　this　study,　a　novel　polysaccharide　TDGP-3　from　Tetrastigma　hemsleyanum　Diels　et　Gilg　was　extracted　by　enzymolysis-ultrasonic　assisted　extraction　(EUAE)　method　and　its　antioxidant　and　hypolipidemic　activities　were　evaluated.　With　a　molecular　weight　of　3.31　x　10(5)　Da,　TDGP-3　was　composed　of　1,4-Glcp,　1,4-Glap　and　1,3,6-Manp　linkage　in　the　main　chain.　TDGP-3　showed　potential　effects　on　relieving　hyperlipidemia　and　preventing　oxidative　stress,　reflecting　by　regulating　blood　lipid　levels　(TC,　TG,　HDL-C　and　LDL-C),　decreasing　the　contents　of　MDA　in　liver,　restoring　the　activities　of　hepatic　antioxidant　enzymes　(SOD,　GSH-Px　and　CAT).　The　results　clearly　indicated　that　the　TDGP-3　possesses　a　potent　hypolipidemic　activity　and　might　be　used　for　hyperlipidaemia　treatment.　(C)　2018　Published　by　Elsevier　B.V.