Three　new　cyclic　lipopeptides　(compounds　1-3)　were　isolated　from　Bacillus　sp.　SY27F,　which　collected　from　the　deep-sea　hydrothermal　vent　in　the　Indian　Ocean.　The　planar　structures　of　compounds　1-3　were　characterized　by　LC-MS,　GC-MS　and　NMR,　whereas　their　absolute　configurations　were　determined　by　modified　Marfey＇s　analysis　and　optical　rotation　contrast.　They　shared　the　same　heptapeptide　chain　(N-L-Gln(1)-D-Leu(2)-L-Leu(3)-L-Val(4)-L-Asp(5)-D-Leu(6)-L-Ile(7)-C),　differing　only　by　the　length　of　the　fatty　acid　chain.　Compounds　1-3　displayed　significant　cytotoxic　activity　against　DU145,　MCF-7　and　HepG2　cancer　cell　lines　with　IC50　values　of　96.8-190.7　nM　and　moderate　to　weak　cytotoxicity　against　A549,　HeLa　and　HT1080,　with　IC50　values　of　9.3-43.2　mu　M,　respectively.　However,　none　of　the　three　compounds　showed　any　antibacterial　activity　against　Escherichia　coli,　Staphylococcus　aureus　and　Monilia　albicans.