Three　new　cyclic　lipopeptides　(compounds　1–3)　were　isolated　from　Bacillus　sp.　SY27F,　which　collected　from　the　deep-sea　hydrothermal　vent　in　the　Indian　Ocean.　The　planar　structures　of　compounds　1–3　were　characterized　by　LC–MS,　GC–MS　and　NMR,　whereas　their　absolute　configurations　were　determined　by　modified　Marfey＇s　analysis　and　optical　rotation　contrast.　They　shared　the　same　heptapeptide　chain　(N-L-Gln1-D-Leu2-L-Leu3-L-Val4-L-Asp5-D-Leu6-L-Ile7-C),　differing　only　by　the　length　of　the　fatty　acid　chain.　Compounds　1–3　displayed　significant　cytotoxic　activity　against　DU145,　MCF-7　and　HepG2　cancer　cell　lines　with　IC50　values　of　96.8–190.7　nM　and　moderate　to　weak　cytotoxicity　against　A549,　HeLa　and　HT1080,　with　IC50　values　of　9.3–43.2　μM,　respectively.　However,　none　of　the　three　compounds　showed　any　antibacterial　activity　against　Escherichia　coli,　Staphylococcus　aureus　and　Monilia　albicans.　©　2019