Developing　high-performance　lateral　flow　immunoassays　(LFIA)　for　portable　and　rapid　profiling　equipment　is　a　key　to　meet　the　field-testing　of　hazardous　pollutants.　Tetrabromobisphenol　A　bis　(2-hydroxyethyl)　ether　(TBBPA-BHEE)　as　an　emerging　hazardous　contaminant　poses　potential　risks　to　human　health,　yet　there　is　no　rapid,　specific　or　sensitive　LFIA　for　monitoring　purpose.　Herein,　a　novel　dual-mode　LFIA　for　field　testing　TBBPA-BHEE　was　pioneered　with　colorimetric/diffuse　reflection　using　gold　nanoflowers　(AuNFs)　and　monoclonal　antibody　(AuNFs@mAb).　Initially,　a　functional　monoclonal　antibody　(mAb)　was　prepared　and　purified.　The　mAb　was　subsequently　labeled　on　AuNFs　to　create　the　AuNFs@mAb　probe,　which　could　be　captured　by　TBBPA-BHEE　molecule　in　10　min　and　achieved　colorimetric/diffuse　reflectance　spectroscopy　using　naked　eyes　or　portable　immunochromato-reader,　respectively.　The　preparation　of　test　strips　was　systematically　studied　and　the　LFIA　for　qualitative　and　quantitative　analysis　of　TBBPA-BHEE　were　evaluated.　The　optimized　LFIA　achieved　a　visual　limit　of　detection　(LOD)　of　5.0　ng/mL　and　a　detection　LOD　of　0.25　ng/mL　by　portable　immunochromato-reader.　It　showed　a　high　specificity　for　TBBPA-BHEE,　good　recovery　yields　of　112.41–120.10　%　with　the　relative　standard　deviation　(RSD)　less　than　8.0　%,　and　excellent　long-term　stability.　Moreover,　the　results　of　the　resultant　LFIA　were　highly　consistent　with　that　of　HPLC　method,　with　a　correlation　coefficient　of　0.995.　Therefore,　this　study　highlights　the　promising　application　in　developing　an　economical　and　selective　LFIA　to　field　profiling　of　TBBPA-BHEE.　©　2025　Elsevier　B.V.