In　this　work,　LaVO4　nanocubes　(LVNCs),　prepared　through　a　facile　hydrothermal　method,　were　fabricated　as　efficient　biomimetic　catalysts,　displaying　exceptional　peroxidase　catalytic　activity.　In　the　presence　of　H2O2,　LVNCs　presented　an　efficient　catalytic　capability　in　promoting　the　oxidation　of　the　colorless　3,3′,5,5′-tetramethylbenzidine　to　its　blue　derivative.　The　catalytic　behavior　of　LVNCs　follows　a　typical　ping-pong　mechanism,　and　its　catalytic　mechanism　can　be　attributed　to　the　fact　that　H2O2　gained　electrons　under　the　catalysis　of　LVNCs　to　generate　strongly　oxidizing　∙O2-.　An　exquisite　colorimetric　platform　based　on　LVNCs　was　thus　constructed　for　the　rapid　detection　of　H2O2　and　glucose.　The　linear　ranges　for　the　quantitative　detection　of　H2O2　and　glucose　were　2–30　μM　and　5–100　μM,　with　the　detection　limits　of　0.067　μM　and　0.83　μM,　respectively.　The　colorimetric　platform　demonstrates　a　superior　catalytic　activity,　remarkable　sensitivity,　resistance　to　interference,　favorable　stability　and　reusability,　and　excellent　practical　samples　testing　utility,　thereby　holding　great　potential　in　complex　environmental　and　food　analysis.　©　2025　Elsevier　B.V.