A　chiral　metal-organic　framework　membrane　was　constructed　through　coordination　between　copper　ions　and　R/S-tryptophan.　The　membrane　exhibits　exceptional　enantioselectivity　towards　amino　acids　(ee　value:　99.38%)　through　synergistic　effects　of　hydrogen　bonding　and　spatial　hindrance.　This　strategy　demonstrates　the　potential　of　amino　acid-based　chiral　centers　for　high-performance　separation　membranes.