Organic　semiconductor　single　crystals　(OSSCs)　exhibit　long-range　intermolecular　order　and　low　defect　density　without　grain　boundaries,　therefore　leading　to　superior　carrier　transport　than　organic　thin-films　but　often　weak　emission　due　to　aggregation-caused　quenching.　Here,　ultrathin　2D　plate-like　OSSCs　of　an　anthracene　derivative,　4,4　＇　-　bis　((E)　-2-　(anthracene-2-yl)　vinyl)　-1,1　＇　-　biphenyl　(BPVAn)　are　designed　and　synthesized,　which　exhibit　not　only　strong　emission　with　a　photoluminescence　quantum　yield　(PLQY)　of　56%　but　also　balanced　and　high　charge　carrier　mobility　of　mu　(h)　=　5.6　cm(2)　V-1　s(-1)　and　mu　(e)　=　1.3　cm(2)　V-1　s(-1)　for　hole　and　electron　transport,　respectively.　Organic　light-emitting　transistors　(OLETs)　based　on　BPVAn　OSSCs　exhibit　strong　and　spatially　controllable　light　emission　within　both　P-　and　N-　conducting　channels,　with　an　external　quantum　efficiency　(EQE)　of　up　to　2.12%　and　a　brightness　of　1115　cd　m(-2).　In　addition,　OSSC　parallelogram　plates　serve　as　whispering　gallery　mode　(WGM)　micro-resonators　and　exhibit　excellent　laser　characteristics,　with　a　low　threshold　of　4.2　mu　J　cm(-2)　and　a　high　quality　(Q)　factor　of　approximate　to　1870　at　an　emission　peak　of　503　nm.　These　results　indicate　that　OSSCs　with　excellent　carrier　transport,　electroluminescence,　and　laser　properties　offer　significant　potential　for　the　further　development　of　electrically　injected　organic　semiconductor　lasers　and　optoelectronic　devices.