Rational:　People　are　widely　exposed　to　parabens　in　their　daily　life,　but　parabens　are　endocrine　disrupting　chemicals　that　pose　a　threat　to　human　health.　Therefore,　establishing　a　rapid　screening　method　to　enhance　monitoring　of　parabens　is　necessary.　Herein,　a　covalent　organic　framework　(COF)　nanofilm-assisted　laser　desorption　ionization　mass　spectrometry　(LDI-MS)　method　was　established　to　screen　parabens　in　personal　care　products　(PCPs).　Methods:　TAPB-TFPB-COF　nanofilm　was　synthesized　on　indium　tin　oxide　(ITO)　glass　and　used　as　LDI-MS　substrates.　To　observe　the　practicability　of　TAPB-TFPB-COF　nanofilm-assisted　LDI-MS,　the　results　of　this　method　for　analyzing　small　molecules　such　as　parabens,　estrogens,　and　bisphenols　were　compared　with　those　of　the　conventional　organic　matrix　9-aminoacridine　(9-AA),　and　the　reproducibility　and　detection　limit　were　further　verified.　Finally,　the　method　was　applied　to　screen　parabens　in　PCPs.　Results:　TAPB-TFPB-COF　nanofilm-assisted　LDI-MS　analyzed　small　molecules　such　as　parabens,　estrogens,　and　bisphenols　with　higher　mass　spectral　signals　and　cleaner　mass　spectral　backgrounds　compared　with　9-AA.　Meanwhile,　the　method　analyzed　methylparaben　(MeP)　with　high　reproducibility　(RSD　=　6.96%)　and　low　detection　limit　(1.64　μM)　and　performed　well　for　rapid　screening　of　parabens　in　PCPs.　Conclusion:　TAPB-TFPB-COF　nanofilm-assisted　LDI-MS　for　analyzing　small　molecules　such　as　parabens,　estrogens,　and　amino　acids　offered　the　advantages　of　rapid　analysis,　a　clean　background,　and　good　reproducibility.　The　method　was　successfully　applied　to　detecting　parabens　in　PCPs,　demonstrating　the　practical　utility　of　LDI-MS　based　on　TAPB-TFPB-COF　nanofilm　for　analyzing　parabens　in　complex　samples.　©　2025　John　Wiley　&　Sons　Ltd.