A　porous　molecular　imprinted　latex　membrane　(MILM)　with　L-Tryptophan　as　template　molecule,　adipic　acid　dihydrazide　(ADH)　as　cross-linker　and　glucose　as　porogen　was　prepared　at　ambient　temperature　from　polyacrylate　core-shell　emulsion　incorporated　with　diacetone　acrylamide　(DAAM)　and　acrylic　acid　(AA).　The　strength　of　interaction　of　functional　monomers　with　L-Tryptophan　was　compared.　The　effects　of　functional　monomers　on　the　separation　properties　of　MILM　were　investigated.　The　results　show　that　the　MILM　with　developed　void　structures　can　be　obtained　by　the　method　proposed　in　this　paper.　The　strength　of　interaction　between　the　three　functional　monomers　and　L-Tryptophan　is　in　the　following　order:　L-Trp/ADH＞L-Trp/DAAM＞L-Trp/AA.　ADH,　AA　and　DAAM　have　considerable　influence　on　crosslinking　degree　and　consequently　the　permeation　properties　of　MILM.　Compared　with　non-imprinted　membrane,　the　acquired　MILM　exhibits　selective　recognition　behavior　towards　template　with　separation　factor　reaching　over　2.0.　©　right.