Lysosomes　are　pivotal　in　diverse　physiological　phenomena,　encompassing　autophagy,　apoptosis,　and　cellular　senescence.　The　demand　for　precise　tumors　treatment　has　led　to　the　development　of　specific　lysosome-targeting　probes　capable　of　elucidating　lysosomal　dynamics　and　facilitating　targeted　cell　death.　In　this　research,　we　report　the　synthesis　and　characterization　of　a　novel　benzopyrrolidinyl-substituted　silicon　phthalocyanine　(Py-SiPc),　designed　for　selective　lysosome　labeling　and　Fluorescence　imaging-guided　in　vitro　photodynamic　therapy.　Furthermore,　we　encapsulated　Py-SiPc　within　a　biocompatible　nanocarrier,　dipalmitoylphosphatidylethanolaminepolyethylene　glycol　2000　(DSPE),　to　create　water-soluble　nanoparticles　(DSPE@Py-SiPc).　These　nanoparticles　exhibit　exceptional　lysosome　labeling　capabilities,　as　evidenced　by　bioimaging　techniques.　Upon　exposure　to　laser　irradiation,　DSPE@Py-SiPc　efficiently　induces　the　production　of　reactive　oxygen　species,　impairing　lysosomal　function　and　triggering　lysosomal-mediated　cell　death.　The　DSPE@Py-SiPc　system　emerges　as　a　promising　photosensitizer.