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author：

Zheng, Z. (Zheng, Z..) [1] | Lin, J. (Lin, J..) [2] | Zhang, J. (Zhang, J..) [3] | Luo, F. (Luo, F..) [4] | Weng, Z. (Weng, Z..) [5] | Wang, J. (Wang, J..) [6] | Lin, C. (Lin, C..) [7] | Qiu, B. (Qiu, B..) [8] | Lin, Z. (Lin, Z..) [9] | Lin, H. (Lin, H..) [10]

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Scopus

Abstract：

Herein,　we　present　the　development　of　an　ultra-sensitive　immobilization-free　homogeneous　electrochemiluminescence　(ECL)　biosensor,　leveraging　the　electrostatic　repulsion　between　a　negatively　charged　indium　tin　oxide　(ITO)　electrode　and　DNA　and　exonuclease　I　(Exo　I)-powered　signal　amplification,　to　achieve　highly　efficient　detection　of　thrombin.　Specifically,　the　aptamer　and　the　complementary　DNA　engage　in　the　formation　of　a　double-stranded　DNA　(dsDNA)　complex.　This　negatively　charged　dsDNA　structure　subsequently　associates　with　a　positively　charged　ECL　indicator,　namely　ruthenium　phenanthroline　(Ru(phen)32+),　resulting　in　the　generation　of　the　dsDNA-Ru(phen)32+　ECL　probe.　The　negatively　charged　dsDNA-Ru(phen)32+　experiences　electrostatic　repulsion　from　the　negatively　charged　ITO　electrode,　resulting　in　a　low　ECL　signal.　Nonetheless,　upon　the　addition　of　thrombin,　the　aptamer　preferentially　binds　to　thrombin,　triggering　the　releases　of　the　embedded　Ru(phen)32+　facilitated　by　Exo　I　and　hence　resulting　in　a　robust　and　enhanced　ECL　signal.　The　amplified　ECL　signal　is　linearly　correlated　with　the　logarithm　of　thrombin　concentration　within　a　detection　range　spanning　from　10　fmol/mL　to　50　pmol/mL,　with　a　remarkable　detection　limit　of　3.21　fmol/mL.　This　strategy　eliminates　the　need　for　cumbersome　labeling　steps,　avoids　the　electrode　modification　process,　overcoming　the　low　immobilization　efficiency　of　aptamers　and　poor　signal　transduction　of　indicators　labeled　at　the　end　of　DNA.　©　2024　Elsevier　B.V.

Keyword：

Biomarker Electrochemiluminescence biosensors Electrostatic force exonuclease I Thrombin

Community：

  • [ 1 ] [Zheng Z.]College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 2 ] [Zheng Z.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 3 ] [Lin J.]College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 4 ] [Lin J.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 5 ] [Zhang J.]College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 6 ] [Zhang J.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 7 ] [Luo F.]College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 8 ] [Luo F.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 9 ] [Weng Z.]College of Biological Science and Engineering, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 10 ] [Wang J.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 11 ] [Lin C.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 12 ] [Qiu B.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 13 ] [Lin Z.]MOE Key Laboratory of Analysis and Detection for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, 350116, China
  • [ 14 ] [Lin H.]Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fujian, Fuzhou, 350116, China

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Source ：

Microchemical Journal

ISSN： 0026-265X

Year： 2024

Volume： 207

4 . 9 0 0

JCR@2023

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SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 2

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