The　precise　biomolecular　detection　is　crucial　in　medical　diagnosis,　biological　research,　and　bioengineering.　In　this　paper,　a　phase　multiplexing　based　molecularly　imprinted　polymer　biosensor　(PM-MIPB)　is　proposed.　It　considers　the　high　throughput　and　non-labeling　detection　mode　by　multiplexing　without　increasing　the　number　of　scanning　and　detecting　elements,　as　well　as　the　high　specificity　and　affinity　of　molecular　imprinting　technology,　to　improve　the　throughput　and　accuracy　of　molecular　detection.　Phase　multiplexing　originates　from　phase-sensitive　spectral-domain　optical　coherence　tomography,　where　the　low-coherence　interferometric　spectra　with　multiple　optical　path　differences　are　spectrally　analyzed　to　identify　and　extract　the　interferometric　phases　across　these　paths.　Based　on　this　principle,　simultaneous　quantitative　detection　and　interaction　analysis　of　multiple　analytes　can　be　realized　by　preparing　multiple　optical-path　difference-encoded　(also　thickness-encoded)　detection　channels　and　simultaneously　extracting　the　phases　of　the　interfering　signals.　Furthermore,　PM-MIPB　utilizes　molecularly　imprinted　polymer　as　an　alternative　to　antigen-antibody　systems,　creating　selective　recognition　sites　through　specific　template　molecules　to　achieve　highly　selective,　specific　and　stable　detection　of　target　molecules.　Theoretically,　PM-MIPB　can　simultaneously　measure　up　to　255　biomolecules,　with　a　detection　speed　of　0.025　seconds,　recovery　rate　ranging　from　95.65%　to　103.41%,　and　sensitivity　of　4.05×10-3　nm·mL/μg.　By　integrating　phase　multiplexing　with　molecular　imprinting,　PM-MIPB　meets　the　high　demand　for　efficient,　high-throughput,　and　accurate　biomolecular　detection　in　life　science　field.　©　2001-2012　IEEE.