Timely　and　rapid　detection　of　antibiotic　residues　in　the　environment　is　conducive　to　safeguarding　human　health　and　promoting　an　ecological　virtuous　cycle.　A　foldable　paper-based　photoelectrochemical　(PEC)　sensor　was　successfully　developed　for　the　detection　of　ampicillin　(AMP)　based　on　glutathione/zirconium　dioxide　hollow　nanorods/aptamer　(GSH@ZrO　2　HS@apt)　modified　cellulose　paper　as　a　reactive　zone　with　laser　direct-writing　lead　sulfide/cadmium　sulfide/graphene　(PbS/CdS/LIG)　as　photoelectrode　and　cobalt　hydroxide　(CoOOH)　as　a　photoresist　material.　Initially,　AMP　was　introduced　into　the　paper-based　reaction　zone　as　a　biogate　aptamer,　which　specifically　recognized　the　target　and　then　left　the　ZrO　2　HS　surface,　releasing　glutathione　(GSH)　encapsulated　inside.　Subsequently,　the　introduction　of　GSH　into　the　reaction　region　and　etching　of　CoOOH　nanosheets　to　expose　the　PbS/CdS/LIG　photosensitive　material　increased　photocurrent.　Under　optimal　conditions,　the　paperbased　PEC　biosensor　showed　a　linear　response　to　AMP　in　the　range　of　5.0　-　2　x　10　4　pM　with　a　detection　limit　of　1.36　pM　(S/N　=　3).　In　addition,　the　constructed　PEC　sensing　platform　has　excellent　selectivity,　high　stability　and　favorable　reproducibility,　and　can　be　used　to　assess　AMP　residue　levels　in　various　real　water　samples　(milk,　tap　water,　river　water),　indicating　its　promising　application　in　environmental　antibiotic　detection.