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author:

Li, Liannishang (Li, Liannishang.) [1] | Gong, Yurong (Gong, Yurong.) [2] | Lin, Qitian (Lin, Qitian.) [3] | He, Shaoying (He, Shaoying.) [4] | Xing, Chao (Xing, Chao.) [5] | Lu, Chunhua (Lu, Chunhua.) [6] (Scholars:卢春华)

Indexed by:

EI Scopus SCIE

Abstract:

Catalytic DNA circuits show great potential for precise intracellular imaging. However, their selectivity and efficiency are often hindered by low anti-interference performance in the cytoplasm 's complex environment. Thus, designing DNA circuits that exhibit enhanced stability and specific activation is crucial for the accurate intracellular biomolecule imaging. Herein, we proposed a framework nucleic acids based endogenously activated entropy-driven catalytic (EDC) circuit, FEED, for in vivo microRNA imaging with enhanced selectivity and efficiency. To mitigate undesired signal leakage before reaching the target cells, the dominating EDC circuitry fuel strand was initially blocked with a disulfide bond modified DNA strand, and the target recognition site of sensing module was also closed by apurinic/apyrimidinic (AP) sites. This configuration allowed selective activation of the EDC by endogenous GSH and human apurinic/apyrimidinic endonuclease 1 (APE1) in cancer cells, facilitating high-contrast miRNA imaging. Additionally, the integrating a distinctive DNA tetrahedral structure into the EDC circuit enhances both biostability and cellular reaction efficacy. This in-site activation FEED circuit offers a programmable and modular amplification strategy for biomarker detection in live cells and mice, providing a potentially valuable molecular tool for living systems.

Keyword:

Catalytic DNA Circuit Entropy-Driven Framework nucleic acids MicroRNA Imaging

Community:

  • [ 1 ] [Li, Liannishang]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 2 ] [Gong, Yurong]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 3 ] [Lin, Qitian]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 4 ] [Xing, Chao]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 5 ] [Lu, Chunhua]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China
  • [ 6 ] [He, Shaoying]Minjiang Univ, Coll Mat & Chem Engn, Fuzhou 350108, Peoples R China
  • [ 7 ] [Xing, Chao]Minjiang Univ, Coll Mat & Chem Engn, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • [Xing, Chao]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China;;[Lu, Chunhua]Fuzhou Univ, Coll Chem, MOE Key Lab Analyt Sci Food Safety & Biol, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350116, Peoples R China;;

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Source :

SENSORS AND ACTUATORS B-CHEMICAL

Year: 2024

Volume: 417

8 . 0 0 0

JCR@2023

Cited Count:

WoS CC Cited Count: 3

SCOPUS Cited Count: 3

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

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