Development　of　rapid　and　low-cost　detection　strategies　to　screen　HPV16　E6　and　E7　oncogenes　are　urgently　needed　for　cervical　cancer　screening.　A　highly　sensitive,　selective　and　label-free　homogeneous　electrochemical　biosensor　for　these　oncogenes　was　developed　in　this　study.　The　assay　combines　the　superior　recognition　ability　of　entropy-driven　amplification　with　the　excellent　signal　amplification　capability　of　hyperbranched　rolling　circle　amplification　(HRCA).　In　the　absence　of　a　target,　most　of　the　electrochemical　indicator,　methylene　blue　(MB),　can　diffuse　freely　onto　the　surface　of　the　negatively　charged　indium　fin　oxide　(ITO)　electrode,　resulting　in　the　production　of　a　distinct　signal.　Conversely,　the　target　DNA　can　trigger　entropy-driven　target　recycling,　causing　the　release　of　multiple　assistant　probe　1,　which　can　generate　numerous　long　strand　ssDNA　and　dsDNA　through　HRCA.　Because　MB　can　embed　into　DNA　fragments　and　the　strong　electrostatic　repulsion　between　long　strand　DNAs　and　the　negative　charged　electrode　causes　a　minimal　amount　of　MB　to　arrive　at　the　electrode　surface,　a　signal　drop　associated　with　the　target　concentration　can　be　observed.　The　electrochemical　response　has　a　linear　relationship　with　the　target　concentration　in　the　range　of　0.1　fM　to　10　pM　with　a　low　detection　limit　of　18.6　attomole　(S/N　=　3).　The　biosensor　was　applied　to　detect　the　target　in　clinical　samples　and　shows　good　consistency　with　the　classical　method.