• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

He, Yinghao (He, Yinghao.) [1] | Cheng, Lingjun (Cheng, Lingjun.) [2] | Yang, Yuanyuan (Yang, Yuanyuan.) [3] | Chen, Ping (Chen, Ping.) [4] | Qiu, Bin (Qiu, Bin.) [5] (Scholars:邱彬) | Guo, Longhua (Guo, Longhua.) [6] | Wang, Yan (Wang, Yan.) [7] | Lin, Zhenyu (Lin, Zhenyu.) [8] (Scholars:林振宇) | Hong, Guolin (Hong, Guolin.) [9]

Indexed by:

EI Scopus SCIE

Abstract:

Development of rapid and low-cost detection strategies to screen HPV16 E6 and E7 oncogenes are urgently needed for cervical cancer screening. A highly sensitive, selective and label-free homogeneous electrochemical biosensor for these oncogenes was developed in this study. The assay combines the superior recognition ability of entropy-driven amplification with the excellent signal amplification capability of hyperbranched rolling circle amplification (HRCA). In the absence of a target, most of the electrochemical indicator, methylene blue (MB), can diffuse freely onto the surface of the negatively charged indium fin oxide (ITO) electrode, resulting in the production of a distinct signal. Conversely, the target DNA can trigger entropy-driven target recycling, causing the release of multiple assistant probe 1, which can generate numerous long strand ssDNA and dsDNA through HRCA. Because MB can embed into DNA fragments and the strong electrostatic repulsion between long strand DNAs and the negative charged electrode causes a minimal amount of MB to arrive at the electrode surface, a signal drop associated with the target concentration can be observed. The electrochemical response has a linear relationship with the target concentration in the range of 0.1 fM to 10 pM with a low detection limit of 18.6 attomole (S/N = 3). The biosensor was applied to detect the target in clinical samples and shows good consistency with the classical method.

Keyword:

DNA sequence Entropy-driven amplification Homogeneous electrochemical biosensor Human papillomavirus Hyperbranched rolling circle amplification

Community:

  • [ 1 ] [He, Yinghao]Xiamen Univ, Dept Lab Med, Xiamen Key Lab Genet Testing, Affiliated Hosp 1, Xiamen 361005, Peoples R China
  • [ 2 ] [Cheng, Lingjun]Xiamen Univ, Dept Lab Med, Xiamen Key Lab Genet Testing, Affiliated Hosp 1, Xiamen 361005, Peoples R China
  • [ 3 ] [Yang, Yuanyuan]Xiamen Univ, Dept Lab Med, Xiamen Key Lab Genet Testing, Affiliated Hosp 1, Xiamen 361005, Peoples R China
  • [ 4 ] [Chen, Ping]Xiamen Univ, Dept Lab Med, Xiamen Key Lab Genet Testing, Affiliated Hosp 1, Xiamen 361005, Peoples R China
  • [ 5 ] [Hong, Guolin]Xiamen Univ, Dept Lab Med, Xiamen Key Lab Genet Testing, Affiliated Hosp 1, Xiamen 361005, Peoples R China
  • [ 6 ] [Wang, Yan]Xiamen Univ, Med Coll, Dept Cardiol, Affiliated Cardiovasc Hosp, Xiamen 361004, Peoples R China
  • [ 7 ] [Qiu, Bin]Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Analyt Sci Food Safety & Biol,Minist Educ, Fuzhou 350116, Fujian, Peoples R China
  • [ 8 ] [Guo, Longhua]Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Analyt Sci Food Safety & Biol,Minist Educ, Fuzhou 350116, Fujian, Peoples R China
  • [ 9 ] [Lin, Zhenyu]Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Analyt Sci Food Safety & Biol,Minist Educ, Fuzhou 350116, Fujian, Peoples R China

Reprint 's Address:

  • 林振宇

    [Lin, Zhenyu]2 Xue Yuan Rd, Fuzhou, Fujian, Peoples R China;;[Hong, Guolin]55 Zhenhai Rd, Xiamen, Fujian, Peoples R China;;[Wang, Yan]2999 Jinshan Rd, Xiamen, Fujian, Peoples R China

Show more details

Related Keywords:

Source :

SENSORS AND ACTUATORS B-CHEMICAL

ISSN: 0925-4005

Year: 2020

Volume: 320

7 . 4 6

JCR@2020

8 . 0 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:160

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count: 39

SCOPUS Cited Count: 43

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Online/Total:89/10009124
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1