It　is　crucial　that　simple　and　high-throughput　methods　for　determining　multiple,　or　groups　of,　sulfonamides　(SAs)　be　developed　since　they　are　widely　used　in　animal　husbandry　and　aquaculture.　We　developed　a　paper-based　multicolor　colorimetric　aptasensor　to　detect　3　SAs:　sulfaquinoxaline　(SQ),　sulfamethoxypyridazine　(SMP)　and　sulfamethoxydiazine　(SMD).　Using　a　broad-specificity　aptamer　as　a　bioreceptor,　we　reduced　the　growth　of　nicotinamide　adenine　dinucleotide　I　(NADH)–ascorbic　acid　(AA)-mediated　gold　nanobipyramids　(AuNBPs)　to　generate　a　multicolor　signal.　We　also　used　a　paper-based　analytical　device　(PAD)　system　to　deposit　AuNBPs　for　a　sensitive　color　signal　read　out.　The　aptasensor　can　detect　more　color　changes　corresponding　to　the　concentrations　of　SQ,　SMP　and　SMD　and　has　higher　sensitivity,　better　specificity　and　stability.　It　can　also　be　used　to　determine　SQ,　SMP　and　SDM　individually,　or　collectively,　or　any　two　together　with　a　visual　detection　limit　of　0.3–1.0　µM,　a　spectrometry　quantification　limit　(LOQ)　of　0.3–0.5　µM　and　a　spectrometry　detection　limits　(LOD)　of　0.09–0.15　µM.　The　aptasensor　was　successfully　used　to　determine　SQ,　SMP　and　SDM　in　fish　muscle　with　a　recovery　of　89–94%　and　a　RSD　n　=　5)　©　2023　by　the　authors.