Optical Label-Free Aptasensor Based on Weak Value Amplification for Real-Time and Ultrasensitive Detection of IgE - Details

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Abstract：

Allergy　is　a　prevalent　disease,　and　the　potential　allergic　population　is　expanding　with　industrialization　and　changes　in　people’s　living　standards.　Serum　immunoglobulin　E　(IgE)　level　is　one　of　the　critical　indicators　for　determining　allergy.　Here,　we　proposed　a　simple,　real-time　monitoring,　low　chip　cost,　label-free　aptamer　biosensing　strategy　based　on　weak　value　amplification　(WVA)　for　the　quantitative　detection　of　IgE　in　serum　samples,　enabling　early　and　accurate　diagnosis　of　allergic　or　hypersensitive　patients.　The　aptasensor　combined　an　imaging　weak　measurement　system　with　the　high　specificity　of　the　aptamer　for　the　marker　IgE.　By　modifying　the　amino　group　at　the　3-terminal　end,　the　anti-IgE　aptamers　can　attach　to　a　dopamine-modified　prism’s　surface　and　selectively　recognize　IgE　in　human　serum.　In　the　presence　of　IgE,　a　specific　binding　reaction　occurred,　resulting　in　a　change　in　the　refractive　index　of　the　reactive　region’s　surface,　manifested　as　a　change　in　the　light　intensity　of　the　camera　acquired　experimental　images.　As　the　concentration　of　IgE　increased,　the　relative　light　intensity　advanced　sequentially.　The　WVA-aptasensing　strategy　achieved　a　wide　detection　range　of　0.01　ng/mL　to　2　μg/mL　in　phosphate　buffered　saline　buffer,　with　the　resolution　as　low　as　4.3　pg/mL.　IgE　testing　experiments　in　human　serum　have　proved　the　feasibility　of　our　methods　in　detecting　complex　samples.　In　addition,　the　method　specifically　recognized　IgE　without　interference　from　other　proteins.　We　believe　that　our　proposed　sensing　strategy　opens　up　new　possibilities　for　ultrahigh　sensitivity　screening　of　IgE　and　can　be　expanded　to　detecting　other　biomolecules.　©　2024　American　Chemical　Society

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aptasensor human IgE refractive index relative light intensity weak value amplification

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[ 1 ] [Wang B.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 2 ] [Wang B.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 3 ] [Meng L.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 4 ] [Meng L.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 5 ] [Liang G.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 6 ] [Liang G.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 7 ] [Zhang L.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 8 ] [Zhang L.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 9 ] [Xu Y.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 10 ] [Xu Y.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 11 ] [Zhang X.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 12 ] [Zhang X.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 13 ] [Li H.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 14 ] [Li H.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 15 ] [Song Z.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 16 ] [Song Z.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 17 ] [Guo C.]School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou, 350108, China
[ 18 ] [Guan T.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 19 ] [Guan T.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 20 ] [He Y.]Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China
[ 21 ] [He Y.]Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China

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