DNA-based　molecular　amplifiers　offer　significant　promise　for　molecular-level　disease　diagnosis　and　treatment,　yet　tailoring　their　activation　for　precise　timing　and　localization　remains　a　challenge.　Herein,　we＇ve　pioneered　a　dual　activation　strategy　harnessing　external　light　and　internal　ATP　to　create　a　highly　controlled　DNA　logic　amplifier　(FDLA)　for　accurate　miRNA　monitoring　in　cancer　cells.　The　FDLA　was　constructed　by　tethered　the　two　functionalized　catalytic　hairpin　assembly　(CHA)　hairpin　modules　(ATP　aptamer　sealed　hairpin　aH1　and　photocleavable　(PC-linker)　sites　modified　hairpin　pH2)　to　DNA　tetrahedron　(DTN).　The　FDLA　system　incorporates　ATP　aptamers　and　PC-linkers　as　logic　control　units,　allowing　them　to　respond　to　both　exogenous　UV　light　and　endogenous　ATP　present　within　cancer　cells.　This　response　triggers　the　release　of　CHA　hairpin　modules,　enabling　amplified　FRET　miRNA　imaging　through　an　AND-AND　gate.　The　DTN　structure　could　improve　the　stability　of　FDLA　and　accelerate　the　kinetics　of　the　strand　displacement　reaction.　It　is　noteworthy　that　the　UV　and　ATP　co-gated　DNA　circuit　can　control　the　DNA　bio-computing　at　specific　time　and　location,　offering　spatial　and　temporal　capabilities　that　can　be　harnessed　for　miRNA　imaging.　Furthermore,　the　miRNA-sensing　FDLA　amplifier　demonstrates　reliable　imaging　of　intracellular　miRNA　with　minimal　background　noise　and　false-positive　signals.　This　highlights　the　feasibility　of　utilizing　both　exogenous　and　endogenous　regulatory　strategies　to　achieve　spatial　and　temporal　control　of　DNA　molecular　circuits　within　living　cancer　cells.　Such　advancements　hold　immense　potential　for　unraveling　the　correlation　between　miRNA　and　associated　diseases.　©　2024　Elsevier　B.V.