Liquid　biopsies　utilizing　tumor　exosomes　offer　a　noninvasive　approach　for　cancer　diagnosis.　However,　validation　studies　consistently　report　that　in　the　early　stages　of　cancer,　the　secretion　of　exosomes　by　cancer　cells　is　relatively　low,　while　bodily　fluids　exhibit　a　high　abundance　of　other　interfering　biomolecules.　Additionally,　target　mutations　or　differences　in　biomarker　expression　among　various　lung　cancer　subtypes　may　contribute　to　detection　failures.　In　this　study,　we　propose　a　targeted　nanoarray-based　early　cancer　diagnostic　approach　for　multiple　subtypes　of　lung　cancer.　The　targeted　nanoarray　was　constructed　by　modifying　five　targeting　aptamers　onto　mesoporous　silica　nanoparticles　through　the　conjugation　between　amino　and　carboxyl　groups.　The　flow　cytometry　experiments　demonstrated　the　specific　recognition　ability　of　the　targeted　nanoarray　to　tumor　exosomes　in　PBS,　even　at　biomarker　expression　levels　as　low　as　1.5　%.　Moreover,　the　TEM　results　indicated　that　the　targeted　nanoarray　could　isolate　tumor　exosomes　in　the　blood　of　tumor-bearing　mice.　Furthermore,　the　targeted　nanoarray　could　detect　tumor　exosomes　in　the　blood　of　various　lung　cancer　bearing　mice,　including　at　the　early　stages　of　cancer,　which　has　just　been　established　for　7　days.　Overall,　the　targeted　nanoarray　represents　a　promising　tool　for　the　early　detection　of　various　subtypes　of　lung　cancer.　©　2024