Drug　repurposing　uses　approved　drugs　as　candidate　anticancer　therapeutics,　harnesses　previous　research　and　development　efforts,　and　benefits　from　available　clinically　suitable　formulations　and　evidence　of　patient　tolerability.　In　this　work,　the　drug　used　clinically　to　treat　chronic　alcoholism,　disulfiram　(DSF),　was　studied　for　its　antitumor　efficacy　in　a　copper-dependent　manner.　The　combination　of　DSF　and　copper　could　achieve　a　tumor　cell　growth　inhibition　effect　comparable　to　those　of　5-fluorouracil　and　taxol　on　head　and　neck　cancer　cells.　Both　bulk　dendrimer　hydrogel　and　microsized　dendrimer　hydrogel　particles　were　utilized　for　the　localized　sustained　release　of　copper　in　the　tumor　site.　The　localized　sustained　release　of　copper　facilitated　the　tumor　inhibition　effect　following　intratumoral　injection　in　a　mouse’s　head　and　neck　cancer　model.　©　2024　American　Chemical　Society