The　behavioral　reasons　for　215　road　transport　accidents　occurring　between　2015　and　2021　were　investigated　to　prevent　road　transport　accidents　with　the　24　Model.　The　direct　causes,　indirect　causes,　radical　causes　and　their　root　causes　were　analyzed　from　the　individual　level　and　organizational　level.　The　results　show　that　the　most　frequent　unsafe　action　is　speeding　(10.67%).　22.33%　of　road　transport　accidents　are　resulted　from　the　managers　illegally　arranging　unqualified　employees　to　work;　Managers　and　leaders　have　a　significant　impact　on　57.67%　of　road　transport　accidents.　The　unsafe　state　caused　by　human　factors　accounted　for　71.53%;　The　lack　of　driver　and　vehicle　safety　management　system　or　implementation　is　particularly　prominent　among　the　root　causes　(20.92%).　Based　on　this,　safety　countermeasures　for　accident　prevention　are　proposed,　and　a　relationship　diagram　of　the　factors　in　the　road　transportation　system　is　constructed.　©　2024　Tsinghua　University　Press.　All　rights　reserved.