Background:　The　fabrication　of　sensors　capable　of　achieving　rapid,　sensitive,　and　highly　selective　detection　of　target　molecules　in　complex　fluids　is　key　to　realizing　their　real-world　applications.　For　example,　there　is　an　urgent　need　in　drugged　driving　roadside　screening　scenarios　to　develop　a　method　that　can　be　used　for　rapid　drug　detection　and　that　avoids　interference　from　the　matrix　in　the　sample.　How　to　minimize　the　interference　of　complex　matrices　in　biofluids　at　the　electrode　interface　is　the　key　to　improve　the　sensitivity　of　the　sensor.　Results:　This　work　develops　a　facile　and　green　method　to　prepare　rough　electrodes　with　a　porous　structure　for　constructing　electrochemical　aptamer-based　(EAB)　sensors　for　rapid,　sensitive　and　accurate　detection　of　Delta(9　)tetrahydrocannabinol　(THC)　in　biofluids.　The　electroactive　area　of　the　rough　electrode　was　21　times　of　smooth　electrode.　And　the　antifouling　performance　of　the　rough　electrode　was　much　better　than　that　of　smooth　electrode.　Based　on　the　unique　advantages　of　the　rough　electrode,　the　developed　EAB　sensor　achieves　rapid　nanomolar　detection　of　THC　in　undiluted　serum,　undiluted　urine　and　50　%　saliva　with　the　detection　limit　of　5.0　nM,　10　nM　and　10　nM,　respectively.　Moreover,　our　method　possesses　good　reproducibility,　accuracy　and　specificity.　Significance:　The　porous　structure　can　effectively　reduce　the　non-specific　adsorption　and　enhance　the　stability　of　the　signal,　while　the　larger　active　area　can　modify　more　aptamers,　thus　improving　the　sensitivity.　The　detection　limits　of　the　EAB　sensor　were　lower　than　the　cutoff　concentration　of　THC　in　drugged　driving　and　the　measuring　process　was　completed　within　60　s　after　target　addition,　which　makes　the　present　sensors　capable　for　real-world　applications.