Implantable　electrodes　are　crucial　for　stimulation　safety　and　recording　quality　of　neuronal　activity.　To　enhance　their　electrochemical　performance,　electrodeposited　nanostructured　platinum　(nanoPt)　and　iridium　oxide　(IrOx)　have　been　proposed　due　to　their　advantages　of　in　situ　deposition　and　ease　of　processing.　However,　their　unstable　adhesion　has　been　a　challenge　in　practical　applications.　This　study　investigated　the　electrochemical　performance　and　stability　of　nanoPt　and　IrOx　coatings　on　hierarchical　platinum-iridium　(Pt-Ir)　substrates　prepared　by　femtosecond　laser,　compared　with　the　coatings　on　smooth　Pt-Ir　substrates.　Ultrasonic　testing,　agarose　gel　testing,　and　cyclic　voltammetry　(CV)　testing　were　used　to　evaluate　the　coatings’　stability.　Results　showed　that　the　hierarchical　Pt-Ir　substrate　significantly　enhanced　the　charge-storage　capacity　of　electrodes　with　both　coatings　to　more　than　330　mC/cm2,　which　was　over　75　times　that　of　the　smooth　Pt-Ir　electrode.　The　hierarchical　substrate　could　also　reduce　the　cracking　of　nanoPt　coatings　after　ultrasonic,　agarose　gel　and　CV　testing.　Although　some　shedding　was　observed　in　the　IrOx　coating　on　the　hierarchical　substrate　after　one　hour　of　sonication,　it　showed　good　stability　in　the　agarose　gel　and　CV　tests.　Stable　nanoPt　and　IrOx　coatings　may　not　only　improve　the　electrochemical　performance　but　also　benefit　the　function　of　neurobiochemical　detection.　©　2023　by　the　authors.