The　utilization　of　modeling　tools　has　gained　significant　attention　recently.　These　models　typically　involve　a　series　of　partial　differential　equations,　which　can　be　challenging　for　novice　modelers.　This　paper　presented　an　approach　in　practical　teaching　of　modeling　tools　for　ion-exchange　chromatography　with　three　parts:　model　introduction,　model　calibration　through　group　learning　using　different　calibration　strategies,　and　model　applications.　This　approach　was　integrated　into　the　traditional　bioseparation　engineering　curriculum　as　an　activity,　using　the　separation　of　monomer-dimer　mixtures　of　monoclonal　antibodies　as　an　example.　Results　of　competitive　group　learning　and　the　Wilcoxon　test　revealed　that　the　parameter-by-parameter　method　was　more　user-friendly　than　the　Yamamoto　method　for　novice　modelers　to　obtain　reasonable　model　parameters　quickly.　Then,　students　used　the　well-fitted　model　for　process　optimization　and　explored　the　effects　of　process　parameters　and　material　input　variation　on　the　chromatography　process,　which　helped　students　appreciate　the　critical　role　of　time　and　material　savings　achieved　through　modeling　tools.　Finally,　the　student　questionnaire　results　revealed　that　over　two-thirds　of　the　students　gave　positive　feedback　on　the　activity.　Through　this　practical　teaching,　students　became　familiar　with　chromatography　modeling　tools,　moving　away　from　the　tedious　formulaic　descriptions　found　in　traditional　modeling　courses.　This　well-designed　activity　can　be　expanded　from　academia　to　industry,　transforming　the　novice　modelers　into　experienced　modelers　who　can　meet　the　high　demands　of　the　modern　biopharmaceutical　industry.　©　2023　American　Chemical　Society　and　Division　of　Chemical　Education,　Inc.