Rapid,　specific,　and　on-site　detection　of　virulent　foodbornepathogenicstrains　plays　a　key　role　in　controlling　food　safety.　In　this　work,an　ultrasensitive　and　specific　Phage@DNAzyme　signal　probe　was　designedto　detect　foodborne　pathogens.　The　proposed　sensing　probe　was　composedof　the　selected　phage　and　functionalized　DNAzyme,　which　realized　thespecific　recognition　of　target　foodborne　pathogens　at　the　strain　leveland　the　efficient　catalysis　of　copper(II)　based　azide-alkyne　cycloaddition(CuAAC)　click　reaction　with　fluorescent　signal,　respectively.　As　aproof　of　concept,　the　virulent　Escherichia　coli　O157:H7　(E.　coli　O157:H7)　as　therepresentative　analyte　was　first　enriched　and　purified　from　the　complexfood　samples　by　a　4-mercaptophenylboronic　acid-modified　gold　slide.Following,　the　Phage@DNAzyme　probes　were　specifically　combined　withthe　captured　E.　coli　O157:　H7　and　catalyzedthe　click　reaction　between　3-azido-7-hydroxycoumarin　and　3-butyn-1-olwith　the　assistance　of　Cu(II)　to　generate　a　visual　fluorescent　signal.Finally,　the　corresponding　fluorescent　signals　were　measured　by　asmartphone　to　quantify　the　target　concentrations.　Under　optimizedconditions,　the　bioassay　exhibited　a　wide　linear　range　from　10(2)　to　10(8)　CFU/mL　and　the　detection　limit　was　50　CFU/mL(S/N　=　3).　It　was　further　extendedto　the　detection　of　another　foodborne　pathogen　Salmonellatyphimurium　with　satisfying　sensing　performances.This　work　gives　a　new　path　for　developing　rapid,　specific,　and　on-sitedetection　methods　for　trace　levels　of　pathogenic　strains　in　foods.