A　uric　acid　(UA)　electrooxidation-based　electrochemical　sensing　sys-tem　is　of　great　interest　in　the　field　of　measurement　science　and　tech-nology;　however,　its　development　is　greatly　plagued　by　the　lack　of　efficient　electrocatalysts,　leading　to　low　detection　sensitivity.　Here-in,　we　report　a　general　method　for　making　a　series　of　metal　single　-atom　catalysts　(SACs)　on　nitrogen-doped　carbon　(M1-CN,　M　=　Mo,　Fe,　Ru,　Mn,　Co,　Cu,　Pt,　Ni)　to　tune　the　rate-determining　step　(RDS)　of　UA　electrooxidation　to　greatly　boost　its　electrocatalytic　perfor-mance.　We　experimentally　demonstrate　that　the　Mo1-CN　shows　the　highest　electrocatalytic　activity　for　UA　electrooxidation　among　the　investigated　M1-CN.　As　a　proof-of-concept　application,　we　construct　a　portable　and　wireless　biosensor　for　high-throughput　detection　of　disease　markers　(human　papillomavirus-16　[HPV-16])　based　on　the　particular　advantage　of　Mo1-CN　in　enhancing　the　UA　electrooxidation　performance.