The　purpose　of　this　study　was　to　investigate　the　effect　of　crimson　snapper　scale　peptides　(CSSPs)　on　H2O2-induced　oxidative　damage　in　Caco-2　cells.　The　levels　of　lactate　dehydrogenase　(LDH)　and　interleukin-8　(IL-8)　in　the　cell　supernatant,　the　activity　of　intracellular　antioxidant　enzymes,　the　release　of　intracellular　reactive　oxygen　species　(ROS)　and　malondialdehyde　(MDA),　and　the　expression　of　apoptosis-related　genes　(Caspase-3,　Caspase-9,　Bax　and　Bcl-2)　were　measured.　The　antioxidant　effects　of　CSSPs　in　intestinal　cells　were　elucidated　from　the　aspects　of　cellular　redox　state　and　apoptosis.　Results　showed　that　CSSPs　mitigated　H2O2-induced　oxidative　damage　in　Caco-2　cells　through　the　antioxidant　defense　system.　This　effect　involved　increased　activities　of　antioxidant　enzymes　and　consequent　inhibition　of　LDH　release,　intracellular　ROS　accumulation,　and　MDA　and　IL-8　production.　Furthermore,　CSSPs　inhibited　oxidative　stress-induced　apoptosis　by　regulating　the　mitochondrial　apoptotic　pathway,　which　significantly　increased　mitochondrial　membrane　potential,　down-regulated　the　mRNA　expression　of　Caspase-3,　Caspase-9　and　Bax,　and　up-regulated　the　mRNA　expression　of　Bcl-2.　In　conclusion,　CSSPs　can　effectively　reduce　the　degree　of　cell　damage　by　maintaining　redox　homeostasis　and　inhibiting　apoptosis.　©　2023　Chinese　Chamber　of　Commerce.　All　rights　reserved.